School of Pharmaceutical Sciences, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250000, China.
School of Pharmaceutical Sciences, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250000, China.
Colloids Surf B Biointerfaces. 2024 Oct;242:114094. doi: 10.1016/j.colsurfb.2024.114094. Epub 2024 Jul 14.
Reactive oxygen species (ROS) provide a promising way to fight bacterial infection and meet the persistent challenge of antibiotic resistance. Nanoenzyme mimics natural enzyme and becomes an effective regulator of ROS level. In this study, NH-MIL-88B with high specific surface area was selected as the core, and the covalent organic skeleton material TP-TA COF was wrapped by "sequential growth" technology. Subsequently, through the second hydrothermal treatment, the inorganic material CuS with excellent photothermal performance was integrated into the outer layer, and the NH-MIL-88B@TP-TA@CuS composite nanoenzyme was synthesized. Different from the traditional nano-enzyme, NH-MIL-88B@TP-TA@CuS nano-enzyme still has good catalytic effect under neutral conditions (pH=7). In addition, NH-MIL-88B@TP-TA@CuS has good near infrared (NIR) absorption rate and high photothermal conversion efficiency (PTCE is 48.7 %), which can be used for photothermal treatment (PTT) of bacteria. Mild photothermal effect can further enhance the enzyme-like catalytic activity of NH-MIL-88B@TP-TA@CuS, so that HO can be more efficiently catalyzed to produce a large number of ROS. The experimental results in vitro show that NH-MIL-88B@TP-TA@CuS can effectively kill Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in the presence of laser irradiation and HO.
活性氧（ROS）为抵抗细菌感染提供了一种很有前景的方法，同时也满足了抗生素耐药性这一持久挑战。纳米酶模拟天然酶，成为调节 ROS 水平的有效调节剂。在本研究中，选择具有高比表面积的 NH-MIL-88B 作为核心，并采用“顺序生长”技术包裹共价有机骨架材料 TP-TA COF。随后，通过第二次水热处理，将具有优异光热性能的无机材料 CuS 整合到外层，合成了 NH-MIL-88B@TP-TA@CuS 复合纳米酶。与传统纳米酶不同，NH-MIL-88B@TP-TA@CuS 纳米酶在中性条件（pH=7）下仍具有良好的催化效果。此外，NH-MIL-88B@TP-TA@CuS 具有良好的近红外（NIR）吸收率和高光热转换效率（PTCE 为 48.7%），可用于细菌的光热治疗（PTT）。温和的光热效应可以进一步增强 NH-MIL-88B@TP-TA@CuS 的酶样催化活性，从而更有效地催化 HO 产生大量的 ROS。体外实验结果表明，在激光照射和 HO 的存在下，NH-MIL-88B@TP-TA@CuS 可以有效地杀死大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）。
