Laboratory of Molecular Medicine, Ordos Central Hospital, Inner Mongolia Autonomous Region, Ordos 017000, China; Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014000, China.
Laboratory of Molecular Medicine, Ordos Central Hospital, Inner Mongolia Autonomous Region, Ordos 017000, China.
Parasitol Int. 2024 Dec;103:102933. doi: 10.1016/j.parint.2024.102933. Epub 2024 Jul 22.
Tick saliva contains a range of critical biological molecules which could inhibit host defenses and guarantee their food supply. Hq023, a novel cDNA sequence, was cloned from a cDNA library constructed from salivary glands of partially-engorged Haemaphysalis qinghaiensis. Hq023 has an open reading frame (ORF) of 408 bp coding a protein containing 135 amino acid residues with a molecular mass of 15 kDa. Database homology showed that Hq023 protein was structurally similar to a natural toxin U33-theraphotoxin-Cg1c from the Chinese tarantula Chilobrachys guangxiensis. A recombinant protein was expressed with the novel cDNA in a prokaryotic system and its analgesic effect was evaluated in mice model. Both tail immersion and hot-plate tests uncovered an antinociceptive activity, while in the acetic acid-induced writhing test this effect was not observed. These results indicated that the novel recombinant protein Hq023 (rHq023) probably possessed a central antinociceptive activity. Finding of the novel protein might pave a new avenue for the development of tick-derived analgesics.
蜱唾液中含有一系列关键的生物分子，这些分子可以抑制宿主防御并保证它们的食物供应。从部分饱血的青海血蜱的唾液腺构建的 cDNA 文库中克隆出一种新型 cDNA 序列 Hq023。Hq023 具有 408bp 的开放阅读框（ORF），编码一种含有 135 个氨基酸残基的蛋白质，分子量为 15kDa。数据库同源性表明，Hq023 蛋白与来自中国狼蛛 Chilobrachys guangxiensis 的天然毒素 U33-theraphotoxin-Cg1c 在结构上相似。新型 cDNA 在原核系统中表达重组蛋白，并在小鼠模型中评估其镇痛作用。尾浸和热板试验均揭示出镇痛活性，而在醋酸诱导的扭体试验中未观察到这种作用。这些结果表明，新型重组蛋白 Hq023（rHq023）可能具有中枢镇痛活性。新型蛋白的发现可能为开发蜱衍生的镇痛药开辟新途径。
