包裹在透明质酸纳米颗粒中的磷酸康普瑞汀A4对口腔鳞状细胞癌具有高度细胞毒性。
Combretastatin A4 phosphate encapsulated in hyaluronic acid nanoparticles is highly cytotoxic to oral squamous cell carcinoma.
作者信息
Sun Chuanxi, Zhou Ziqi, Liu Fangqiang, Li Hong, Liu Zhe
机构信息
Department of Orthodontics, The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi Province, China.
Jiangxi Province Key Laboratory of Oral Biomedicine, Nanchang, Jiangxi Province, China.
出版信息
Arch Med Sci. 2024 Jun 30;20(3):1022-1028. doi: 10.5114/aoms/189535. eCollection 2024.
INTRODUCTION
To investigate the toxicity of combretastatin A4 phosphate (CA4P) hyaluronic acid (HA) gel nanoparticles (HA-CA4P-NPs) in OSCC (oral squamous cell carcinoma).
METHODS
Toxicity was investigated using fluorescence microscopy, MTT assay, flow cytometry, and OSCC xenograft mouse models.
RESULTS
Compared with CA4P, HA-CA4P-NPs generated nearly 10 times more fluorescence in OSCC cells. Cytotoxicity assays showed that HACA4P-NPs were more toxic to SCC-4 cells but not to HNECs. Remarkable necrosis was induced in SCC-4 cells after exposure to HA-CA4P-NPs, and related proteins were upregulated. Furthermore, HA-CA4P-NPs significantly reduced the tumour size.
CONCLUSIONS
HA-CA4P-NPs improved drug release and delivery, and increased cytotoxicity to cancer cells.
引言
研究磷酸考布他汀A4（CA4P）透明质酸（HA）凝胶纳米颗粒（HA-CA4P-NPs）对口腔鳞状细胞癌（OSCC）的毒性。
方法
使用荧光显微镜、MTT法、流式细胞术和OSCC异种移植小鼠模型研究毒性。
结果
与CA4P相比，HA-CA4P-NPs在OSCC细胞中产生的荧光几乎多10倍。细胞毒性试验表明，HA-CA4P-NPs对SCC-4细胞毒性更大，但对人正常口腔角质形成细胞（HNECs）无毒性。暴露于HA-CA4P-NPs后，SCC-4细胞中出现明显坏死，相关蛋白上调。此外，HA-CA4P-NPs显著减小了肿瘤大小。
结论
HA-CA4P-NPs改善了药物释放和递送，并增加了对癌细胞的细胞毒性。
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