Muthukumaran N, Reddi A H
Clin Orthop Relat Res. 1985 Nov(200):159-64.
The sequential cellular changes in the implants in response to collagenous bone matrix-induced local bone formation include: binding of fibronectin to matrix, chemotaxis and attachment of progenitor cells, proliferation and differentiation of progenitor cells into chondrocytes, and finally osteogenesis and marrow differentiation. The cellular origin of osteogenic proteins is not clear. The present study compares the osteogenic potential of demineralized rat and porcine bone matrix by dissociative extraction and reconstitution. Judging from the Sephacryl S-200 gel filtration profiles of the dissociative extracts of rat and porcine matrix, the latter appears to be smaller. Under identical experimental conditions, the rat chondrosarcoma and osteosarcoma were examined for chondrogenic and osteogenic properties and found to be devoid of inductive potential. It is noteworthy that gel filtration fractions of rat chondrosarcoma on Sepharose CL-6B are inhibitory to bone inductive potential of demineralized rat bone matrix.
纤连蛋白与基质结合、祖细胞的趋化性和附着、祖细胞增殖并分化为软骨细胞，最终成骨和骨髓分化。成骨蛋白的细胞来源尚不清楚。本研究通过解离提取和重组比较了脱矿大鼠和猪骨基质的成骨潜力。从大鼠和猪基质解离提取物的Sephacryl S-200凝胶过滤图谱判断，后者似乎较小。在相同实验条件下，对大鼠软骨肉瘤和骨肉瘤的软骨生成和成骨特性进行了检测，发现它们没有诱导潜力。值得注意的是，大鼠软骨肉瘤在Sepharose CL-6B上的凝胶过滤级分对脱矿大鼠骨基质的骨诱导潜力具有抑制作用。
