与细胞外囊泡相关的补体膜攻击复合物蛋白的定量分析

Quantitative Analysis of Complement Membrane Attack Complex Proteins Associated with Extracellular Vesicles.

作者信息

Turko Illarion V

机构信息

Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, University of Maryland, Rockville, ML 20850, USA.

出版信息

Proteomes. 2024 Jul 12;12(3):21. doi: 10.3390/proteomes12030021.

DOI:10.3390/proteomes12030021

PMID:39051239

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11270256/

Abstract

Extracellular vesicles (EVs) represent a universal mechanism of intercellular communication in normal and pathological conditions. There are reports showing the presence of complement proteins in EV preparations, specifically those that can form a membrane attack complex (MAC). In the present work, we have used a quantitative mass spectrometry method that allows for the measurement of multiple targeted proteins in one experimental run. The quantification of MAC-forming proteins, namely C5b, C6, C7, C8, and C9, in highly purified EVs from normal human plasma revealed the presence of MAC proteins at approximately equal stoichiometry that does not fit the expected stoichiometry of preformed MAC. We concluded that while MAC proteins can be associated with EVs from normal plasma and presumably can be delivered to the recipient cells, there is no evidence that the EVs carry preformed MAC.

摘要

细胞外囊泡（EVs）是正常和病理条件下细胞间通讯的一种普遍机制。有报道显示在EV制剂中存在补体蛋白，特别是那些能够形成膜攻击复合物（MAC）的蛋白。在本研究中，我们使用了一种定量质谱方法，该方法能够在一次实验运行中测量多种靶向蛋白。对来自正常人血浆的高度纯化的EV中形成MAC的蛋白，即C5b、C6、C7、C8和C9进行定量分析，结果显示MAC蛋白以大致相等的化学计量比存在，这与预先形成的MAC的预期化学计量比不符。我们得出的结论是，虽然MAC蛋白可以与正常血浆中的EV相关联，并且大概可以传递给受体细胞，但没有证据表明EV携带预先形成的MAC。