Hernández-Rizo Laura, Villanueva-Martínez Angélica, Salas-Téllez Enrique, Camacho-Enríquez Brígida Del Carmen, Vargas-Martínez María Gabriela, Ganem-Rondero Adriana
Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Av. 1° de Mayo S/N Cuautitlán Izcalli, Estado de México 54740, México.
Laboratorio 17, Inmunodiagnóstico microbiológico, Unidad de investigación Multidisciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54714, México.
Pharm Nanotechnol. 2024 Jul 24. doi: 10.2174/0122117385315666240708060501.
Since wounds are a primary source of infection, it is desirable to have a wound dressing that prevents infectious processes during the tissue regeneration phase. In this regard, silver nanoparticles, oregano essential oil, and chitosan have been utilized due to their antimicrobial activity. This work focused on the preparation of a composite containing these three components, intended to provide protection for wounds, especially by exerting antimicrobial effects.
A composite based on chitosan nanoparticles loaded with oregano essential oil (OEO) and silver nanoparticles was fabricated through the casting-solvent evaporation method. The films were prepared from a suspension of chitosan nanoparticles. The nanoparticles were characterized by size and entrapment efficiency. The surface of the films was observed by SEM, and the mechanical resistance, occlusive capacity, and antimicrobial activity against S. aureus, E. coli, and P. aeruginosa were evaluated. The release of OEO from the films was studied using Franz-type cells.
A composite was successfully prepared from a dispersion of OEO-loaded chitosan nanoparticles (147.8 nm, PDI = 0.35; entrapment efficiency = 80.9 %; loading capacity = 38 %) and silver nanoparticles (19.6 nm, PDI = 0.4). A film could be formed that made the composite by pouring the chitosan nanoparticle dispersion directly into molds. The composite presented advantageous characteristics, such as being semi-occlusive (occlusion factor ~ 40 % and reduction in TEWL of 18 %), allowing the sustained release of OEO (about 0.2 mgCm-2 h-1 during 8 h), and having antimicrobial activity for the three strains evaluated.
The prepared composite can be considered a potential candidate for dressing materials intended to prevent and treat wound infections.
由于伤口是感染的主要来源，因此需要一种在组织再生阶段能预防感染过程的伤口敷料。在这方面，银纳米颗粒、牛至精油和壳聚糖因其抗菌活性而被使用。这项工作专注于制备包含这三种成分的复合材料，旨在为伤口提供保护，特别是通过发挥抗菌作用。
通过流延-溶剂蒸发法制备了一种基于负载牛至精油（OEO）的壳聚糖纳米颗粒和银纳米颗粒的复合材料。薄膜由壳聚糖纳米颗粒悬浮液制备而成。对纳米颗粒进行了粒径和包封率表征。通过扫描电子显微镜观察薄膜表面，并评估其机械抗性、封闭能力以及对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的抗菌活性。使用弗兰兹型扩散池研究了OEO从薄膜中的释放情况。
成功地从负载OEO的壳聚糖纳米颗粒（147.8 nm，多分散指数 = 0.35；包封率 = 80.9%；载药量 = 38%）和银纳米颗粒（19.6 nm，多分散指数 = 0.4）的分散体制备出复合材料。通过将壳聚糖纳米颗粒分散液直接倒入模具中可形成使复合材料成膜。该复合材料具有诸如半封闭（封闭因子约为40%，经皮水分流失降低18%）、允许OEO持续释放（8小时内约为0.2 mg·cm-2·h-1）以及对所评估的三种菌株具有抗菌活性等优势特性。
所制备的复合材料可被视为预防和治疗伤口感染的敷料材料的潜在候选物。
