Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus 22060, Pakistan.
College of Pharmacy and Health Sciences, Ajman University, Ajman 346, United Arab Emirates.
Int J Pharm. 2019 Jun 10;564:22-38. doi: 10.1016/j.ijpharm.2019.04.046. Epub 2019 Apr 16.
The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique. In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer. The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis. The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected. The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains. The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h. The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates). The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
本研究报告了壳聚糖-丝胶-载银纳米复合材料（CSSN）薄膜的制备和评价，其中包括载有莫西沙星（Mox）和未载药的 CSSN 薄膜。
采用原位合成法，将银纳米粒子原位合成到壳聚糖-丝胶胶体复合物中，然后通过溶剂浇铸法制备成薄膜。通过紫外可见分光光度计和zeta 粒径仪对银纳米粒子的原位形成和粒径分析。对制备的薄膜进行溶胀率、含量均匀性、体外 Mox 释放和渗透分析。对复合材料的形态（SEM）、元素（EDX）、光谱（FT-IR）、结构（XRD）和热性能（TGA 和 DSC）进行了检测。还对 CSSN 薄膜的抗菌活性进行了评价，针对七种包括五种 ATCC 和两种临床分离株的致病菌进行了测试。评价了复合膜对 SD 雄性大鼠烧伤模型的潜在伤口愈合活性。
所制备的薄膜具有良好的溶胀性能，具有持续的体外 Mox 释放和渗透性能；在 24 小时内达到最大释放率 78.57%（CSSM3）和最大渗透率 55.05%（CSSM1）。所制备的薄膜，特别是载药的 CSSN 薄膜，对所有测试菌株均表现出良好的抗菌活性，对 MRSA（临床分离株）的活性最高。所制备的薄膜在 7 天的治疗后显示出显著的伤口愈合应用潜力，纤维化、胶原重组、新生血管形成和轻度表皮再生，动物血液中未检测到银离子。
研究结果强烈表明，可将所制备的新型复合敷料用于伤口护理应用。
