Song Zixin, Geng Jiman, Wang Dongdong, Fang Jiaxuan, Wang Ziwen, Wang Changtao, Li Meng
College of Light Industry Science and Engineering, Beijing Technology & Business University, 11 Fucheng Road, Haidian District, Beijing, 100048, China.
Beijing Key Laboratory of Plant Resource Research and Development, Beijing Technology and Business University, 11 Fucheng Road, Beijing, 100048, China.
Bioresour Bioprocess. 2024 Jul 25;11(1):73. doi: 10.1186/s40643-024-00792-2.
Human immortal keratinocyte cells (HaCaT) are induced with UVB to establish an injury model. This model is utilized to investigate whether oat bran fermentation broth (OBF) has a reparative effect on skin inflammation and damage to the skin barrier caused by UVB irradiation. The results show that compared with unfermented oat bran (OB), OBF exhibits higher structural homogeneity, increased molecular weight size, active substances content, and in vitro antioxidant activity. OBF has a scavenging effect on excess reactive oxygen species (ROS) and increases the intracellular levels of antioxidant enzymes. It was found that OBF has a stronger inhibitory effect on the release of inflammatory factors than OB. It increases the synthesis of AQP3 and FLG proteins while decreasing the secretion of KLK-7. OBF can inhibit the transcription level of inflammatory factors by suppressing the JAK/STAT signaling pathway. Safety experiments demonstrate that OBF has a high safety profile.
用人永生化角质形成细胞（HaCaT）经紫外线B（UVB）诱导建立损伤模型。利用该模型研究燕麦麸发酵液（OBF）对UVB照射引起的皮肤炎症和皮肤屏障损伤是否具有修复作用。结果表明，与未发酵燕麦麸（OB）相比，OBF具有更高的结构均匀性、更大的分子量、更高的活性物质含量和体外抗氧化活性。OBF对过量活性氧（ROS）具有清除作用，并提高细胞内抗氧化酶水平。发现OBF对炎症因子释放的抑制作用比OB更强。它增加水通道蛋白3（AQP3）和丝聚蛋白（FLG）的合成，同时减少激肽释放酶-7（KLK-7）的分泌。OBF可通过抑制JAK/STAT信号通路来抑制炎症因子的转录水平。安全性实验表明，OBF具有较高的安全性。
