通过整合磷酸化蛋白质组和互作组分析系统地鉴定激酶-底物关系。
Systematic Identification of Kinase-Substrate Relationship by Integrated Phosphoproteome and Interactome Analysis.
机构信息
Laboratory of Proteomics for Drug Discovery, Center for Drug Design Research, National Institute of Biomedical Innovation, Osaka, Japan.
Laboratory of Proteomics and Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
出版信息
Methods Mol Biol. 2024;2823:11-25. doi: 10.1007/978-1-0716-3922-1_2.
The sensitivity of phosphorylation site identification by mass spectrometry (MS)-based phosphoproteomics has improved significantly. However, the lack of kinase-substrate relationship (KSR) data has hindered improvement of the range and accuracy of kinase activity prediction using phosphoproteome data. We herein describe the application of a systematic identification of KSR by integrated phosphoproteome and interactome analysis using doxycycline (Dox)-induced target kinase-overexpressing HEK-293 cells.
基于质谱（MS）的磷酸化蛋白质组学在磷酸化位点鉴定方面的灵敏度有了显著提高。然而，激酶-底物关系（KSR）数据的缺乏阻碍了利用磷酸蛋白质组数据提高激酶活性预测的范围和准确性。本文描述了通过使用强力霉素（Dox）诱导的靶激酶过表达 HEK-293 细胞，进行整合磷酸蛋白质组和相互作用组分析，对 KSR 进行系统鉴定的应用。
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