Center for Cell and Gene Therapy, Clinical Research Center for Cell-based Immunotherapy, Shanghai Pudong Hospital, State Key Laboratory of Genetic Engineering, School of Life Sciences, Human Phenome Institute , Fudan University, Shanghai, China.
State Key Laboratory of Cell Differentiation and Regulation, Henan International Joint Laboratory of Pulmonary Fibrosis, Henan center for outstanding overseas scientists of pulmonary fibrosis, College of Life Science, Institute of Biomedical Science, Henan Normal University, Xinxiang, Henan, China.
Methods Mol Biol. 2024;2823:109-127. doi: 10.1007/978-1-0716-3922-1_8.
Microphthalmia transcription factor (MiT) family translocation renal cell carcinoma (tRCC) is a rare, aggressive, and heterogeneous subtype of kidney cancer, which is not well characterized. Since genetic alterations are always associated with carcinogenesis, and proteins are the major executors of biological features, multi-omics studies can reveal the systematic tRCC biological process comprehensively. Here, we describe the proteogenomic workflow for characterization of tRCC in detail to provide the knowledge foundation for integrated proteogenomic analysis of tRCC and other malignant tumors in the future.
小眼畸形转录因子（MiT）家族易位肾细胞癌（tRCC）是一种罕见的、侵袭性的、异质性的肾癌亚型，其特征尚未完全阐明。由于遗传改变总是与癌变相关，而蛋白质是生物特征的主要执行者，多组学研究可以全面揭示 tRCC 的系统生物学过程。在这里，我们详细描述了 tRCC 的蛋白质基因组学工作流程，为未来 tRCC 及其他恶性肿瘤的综合蛋白质基因组学分析提供了知识基础。
