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[大肠杆菌和酵母的生物质、半成品及制剂中核酸和蛋白质的不同测量方法比较]

[Comparison of different methods for measuring nucleic acids and protein in the biomass, half-products and preparations of E. coli and yeast].

作者信息

Pupkova V I, Bakhalov O V, Marich T A

出版信息

Prikl Biokhim Mikrobiol. 1979 Jul-Aug;15(4):605-11.

PMID:390531
Abstract

In the E. coli biomass, half-products and RNA preparations a comparative measurement of nucleic acids and protein was carried out using different methods. As a result, the following methods can be recommended: to assay RNA in the biomass--spectrophotometry, to determine RNA in half-products and preparations--the phosphate method, to assess DNA--the diphenyl amine method, and to measure protein--according to Lowry. The same methods can be recommended to estimate RNA from baker's yeast.

摘要

对大肠杆菌的生物质、半成品和RNA制剂,使用不同方法对核酸和蛋白质进行了比较测量。结果表明,可推荐以下方法:测定生物质中的RNA——分光光度法,测定半成品和制剂中的RNA——磷酸盐法,评估DNA——二苯胺法,以及测量蛋白质——根据洛瑞法。估算面包酵母中的RNA时也可推荐相同的方法。

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