Gordon S H, Greene R V, Freer S N, James C
United States Department of Agriculture, Northern Regional Research Center, Peoria, Illinois 61604.
Biotechnol Appl Biochem. 1990 Feb;12(1):1-10.
A relatively new analytical technique, Fourier transform infrared-photoacoustic spectroscopy (FTIR-PAS), provides spectra of bacteria, fungi, and other microorganisms in solid states not suitable for conventional absorption spectroscopy. In this paper the feasibility of quantitative measurement of protein biomass on solid substrates by FTIR-PAS is examined and discussed. By measuring photoacoustic absorption bands from amide groups in the protein of microorganisms, the increase in biomass that occurs during growth was monitored directly and accurately. Incorporation of polyacrylonitrile into the sample as an internal standard was shown to be a convenient method for improving both the reliability and the range of detection by photoacoustic spectroscopy. Results of FTIR-PAS measurements of known quantities of microbial mass in simulated growth experiments suggest that the technique may be especially suitable for assays of microorganisms used in solid-state biosyntheses of drugs, hormones, and other biological agents.
傅里叶变换红外光声光谱法（FTIR-PAS）是一种相对较新的分析技术，它能为细菌、真菌及其他微生物提供固态光谱，而这些固态样品并不适合传统的吸收光谱分析。本文对利用FTIR-PAS定量测量固体基质上蛋白质生物量的可行性进行了研究和讨论。通过测量微生物蛋白质中酰胺基团的光声吸收带，可直接且准确地监测生长过程中生物量的增加。结果表明，将聚丙烯腈作为内标物加入样品中，是一种提高光声光谱法检测可靠性和检测范围的便捷方法。在模拟生长实验中对已知量微生物质量进行FTIR-PAS测量的结果表明，该技术可能特别适用于对用于药物、激素及其他生物制剂固态生物合成的微生物进行检测。
