Research Center for High Altitude Medicine, Key Laboratory for High Altitude Medicine, Ministry of Education, Qinghai University, Xining, 810016, China; Qinghai Provincial People's Hospital, Xining, 810000, China.
Department of Animal Science, College of Animal Science, Xinjiang Agricultural University, Urumqi, 830052, China.
Biochem Biophys Res Commun. 2024 Nov 12;733:150350. doi: 10.1016/j.bbrc.2024.150350. Epub 2024 Jul 9.
Postovulatory aging of oocytes involves a series of deleterious molecular and cellular changes, which adversely affect oocyte maturation, fertilization, and early embryonic development. Petunidin-3-O-(6-O-pcoumaroyl)-rutinoside-5-O-glucoside (PrG), the main active ingredient of anthocyanin, exerts antioxidant effects. This study investigated whether PrG supplementation could delay postovulatory oocyte aging by alleviating oxidative stress. Our results showed that PrG supplementation decreased the number of abnormal morphology oocytes and improved the oxidative stress of aged oocytes by facilitating the reduction of the reactive oxygen species, the increase in glutathione content, and the recovery of expression of antioxidant-related gene expression. In addition, PrG treatment recovered mitochondrial dysfunction, including mitochondrial distribution, mitochondrial membrane potential and adenosine triphosphate in aged oocytes. PrG-treated oocytes returned to normal levels of cytoplasmic and mitochondrial calcium. Notably, PrG inhibited early apoptosis in aged oocytes. RNA-seq and qRT-PCR results revealed that PrG ameliorated oxidative stress injury in postovulatory aging oocytes of mice via the putrescine pathway. In conclusion, in vitro PrG supplementation is a potential therapy for delaying postovulatory oocyte aging.
卵母细胞的排卵后老化涉及一系列有害的分子和细胞变化，这些变化会对卵母细胞成熟、受精和早期胚胎发育产生不利影响。锦葵素-3-O-(6-O-对香豆酰基)-芦丁-5-O-葡萄糖苷（PrG）是花色苷的主要活性成分，具有抗氧化作用。本研究探讨了 PrG 补充是否可以通过减轻氧化应激来延缓排卵后卵母细胞的衰老。我们的结果表明，PrG 补充可以通过减少活性氧的产生、增加谷胱甘肽的含量和恢复抗氧化相关基因的表达来减少异常形态卵母细胞的数量，改善老化卵母细胞的氧化应激。此外，PrG 处理恢复了线粒体功能障碍，包括老化卵母细胞中线粒体的分布、线粒体膜电位和三磷酸腺苷。PrG 处理的卵母细胞细胞质和线粒体钙水平恢复正常。值得注意的是，PrG 抑制了老化卵母细胞的早期凋亡。RNA-seq 和 qRT-PCR 结果表明，PrG 通过腐胺途径改善了小鼠排卵后老化卵母细胞的氧化应激损伤。总之，体外补充 PrG 是一种延缓排卵后卵母细胞衰老的潜在治疗方法。
