Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Aug 15;1244:124240. doi: 10.1016/j.jchromb.2024.124240. Epub 2024 Jul 24.
An imidacloprid colloidal gold immunochromatographic strip was developed in this work, and systematic analytical conditions were deeply investigated. The test strips were used for rapid screening of imidacloprid residues in Chinese herbal medicines. The performance of the colloidal gold test strips was investigated by using five selected Chinese herbal medicines (malt, Coix seed, lotus seed, dried ginger and honeysuckle). As a result, the developed imidacloprid colloidal gold immunochromatographic test strips could be used for rapid screening of imidacloprid residues in 60 kinds of different herbs (including 26 kinds of root/rhizome medicines, 20 kinds of seed/fruit/pericarp medicines, 11 kinds of flower/leaf/whole herb medicines, and 3 kinds of bark/aboveground issues of herb medicines), and the cut-off value was 50 μg/kg. The development of this method can achieve the goal of on-site, rapid and low-cost screening of imidacloprid residues in different herbs, which is of great significance for the quality assurance of herbs.
本工作研制了一种吡虫啉胶体金免疫层析试纸条，并对其系统的分析条件进行了深入研究。该试纸条用于快速筛查中草药中的吡虫啉残留。通过使用五种选定的中草药（麦芽、薏苡仁、莲子、干姜和金银花）来研究胶体金测试条的性能。结果表明，所研制的吡虫啉胶体金免疫层析检测试纸条可用于快速筛查 60 种不同草药（包括 26 种根茎类药物、20 种种子/果实/果皮类药物、11 种花/叶/全草类药物和 3 种树皮/地上部分草药）中的吡虫啉残留，其截断值为 50μg/kg。该方法的开发可以实现现场、快速和低成本筛查不同草药中吡虫啉残留的目标，这对于草药的质量保证具有重要意义。
