College of Biology, Food & Environment, Hefei University, Hefei 230601, PR China.
College of Biology, Food & Environment, Hefei University, Hefei 230601, PR China; Key Laboratory of Berry Processing and Resource Comprehensive Utilization, Hefei University, Hefei 230601, PR China.
Food Chem. 2024 Dec 1;460(Pt 1):140493. doi: 10.1016/j.foodchem.2024.140493. Epub 2024 Jul 24.
In this study, the structural characterization, physicochemical properties, antioxidant, hypolipidemic, and hypoglycemic potentials of polysaccharide components (BLP-1, BLP-2, and BLP-3) purified from blueberry leaf polysaccharides (BLP) were investigated. Ion chromatography results showed that BLP-1, BLP-2, and BLP-3 contained rhamnose, arabinose, galactose, glucose, and glucuronic acid. In contrast to BLP-1, BLP-2 and BLP-3 included galacturonic acid. The methylation analysis results indicated that the backbones of BLP-1, BLP-2, and BLP-3 were mainly composed of glycosidic linkages of arabinose, galactose, and glucose, which was consistent with the results of the previously determined monosaccharide composition. The in-vitro antioxidant results showed that BLP-1, BLP-2, and BLP-3 possessed antioxidant activity with the highest scavenging of -OH radicals. Furthermore, BLP-1, BLP-2, and BLP-3 showed high bile acid-binding activity and α-amylase inhibitory activity, suggesting that they have the potentials of hypolipidemic and hypoglycemic. This study provides a reference for the utilization of blueberry leaf resources.
本研究对从蓝莓叶多糖（BLP）中纯化得到的多糖组分（BLP-1、BLP-2 和 BLP-3）进行了结构表征、理化性质、抗氧化、降血脂和降血糖潜力的研究。离子色谱结果表明，BLP-1、BLP-2 和 BLP-3 含有鼠李糖、阿拉伯糖、半乳糖、葡萄糖和葡萄糖醛酸。与 BLP-1 不同，BLP-2 和 BLP-3 还含有半乳糖醛酸。甲基化分析结果表明，BLP-1、BLP-2 和 BLP-3 的骨架主要由阿拉伯糖、半乳糖和葡萄糖的糖苷键组成，这与先前确定的单糖组成结果一致。体外抗氧化结果表明，BLP-1、BLP-2 和 BLP-3 具有抗氧化活性，对 -OH 自由基的清除能力最强。此外，BLP-1、BLP-2 和 BLP-3 表现出较高的胆汁酸结合活性和α-淀粉酶抑制活性，提示其具有降血脂和降血糖的潜力。本研究为蓝莓叶资源的利用提供了参考。
