College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
Ultrason Sonochem. 2024 Dec;111:107085. doi: 10.1016/j.ultsonch.2024.107085. Epub 2024 Sep 26.
Adhere to the concept of low-carbon environmental protection and turning waste into treasure, polysaccharides from Flammulina velutipes residue polysaccharide (FVRP) has been developed and possesses diverse bioactivities, comprising antioxidant, hypoglycemic, and relieving heavy metal damage, which still has the disadvantages of high molecular weight and low bioavailability. The current work is the first to prepare a degraded polysaccharide (FVRPV) from FVRP by ultrasonic assisted HO-Vc technique in order to reduce its molecular weight, thereby improving its activity and bioavailability. Our results found that the molecular weight and average particle size were declined, but the monosaccharide composition and characteristic functional group types of FVRPV had no impact. The structural changes of polysaccharides analyzed by XRD, Congo Red test, I-KI, SEM, and methylation analysis indicated that the surface morphology and glycosidic bond composition of FVRPV possessed longer side chains and a greater number of branches with an amorphous crystal structure devoid of a triple helix configuration, and had experienced notable alterations after ultrasonic assisted HO-Vc treatment. Meanwhile, the in vitro antioxidant capacity of FVRPV had significantly increased compared to FVRP, implying ultrasonic assisted HO-Vc technique maybe a effective method to enhance the bioactivity of polysaccharides. In addition, the content of polysaccharide, reducing sugar, and uronic acid in FVRPV was significantly decreased, but antioxidant capacity of fermentation broth was stronger by in vitro human fecal fermentation. The 16S rDNA sequencing data displayed that FVRPV can enrich probiotics and reduce the abundance of pathogenic bacteria through different metabolic pathways mediated by gut microbiota, thereby exerting its potential probiotic effects. The interesting work provides a novel degraded polysaccharide by ultrasonic assisted HO-Vc technique, laying a foundation for developing FVRPV as a new antioxidant and prebiotic.
坚持低碳环保、变废为宝的理念，从金针菇菇渣中开发出具有多种生物活性的多糖——金针菇菇渣多糖（FVRP），包括抗氧化、降血糖、缓解重金属损伤等作用，但仍存在分子量高、生物利用度低的缺点。本研究首次采用超声辅助 HO-Vc 技术制备 FVRP 的降解多糖（FVRPV），以降低其分子量，从而提高其活性和生物利用度。结果发现，FVRPV 的分子量和平均粒径减小，但单糖组成和特征功能基团类型没有变化。通过 XRD、刚果红试验、I-KI、SEM 和甲基化分析对多糖的结构变化进行分析，表明 FVRPV 的表面形态和糖苷键组成具有更长的侧链和更多的支链，呈无定形晶体结构，没有三螺旋构象，超声辅助 HO-Vc 处理后发生了明显的变化。同时，FVRPV 的体外抗氧化能力比 FVRP 显著提高，表明超声辅助 HO-Vc 技术可能是提高多糖生物活性的有效方法。此外，FVRPV 中的多糖、还原糖和糖醛酸含量显著降低，但发酵液的体外抗氧化能力通过人粪便发酵增强。16S rDNA 测序数据显示，FVRPV 可以通过肠道微生物群介导的不同代谢途径富集益生菌，减少致病菌的丰度，从而发挥其潜在的益生菌作用。这项有趣的工作提供了一种新的超声辅助 HO-Vc 技术降解多糖，为开发 FVRPV 作为新型抗氧化剂和益生元奠定了基础。
