School of Food Science and Engineering, State Key Laboratory of Biobased Material and Green Papermaking, School of Materials Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
Huzhou Key Laboratory of Materials for Energy Conversion and Storage, School of Science, Huzhou University, Zhejiang, Huzhou 313000, China.
Food Chem. 2024 Nov 15;458:140495. doi: 10.1016/j.foodchem.2024.140495. Epub 2024 Jul 17.
Herein, a signal stable molecularly imprinted photoelectrochemical (MIP-PEC) sensing platform was designed to sensitively detect Escherichia coli by incorporating polythiophene film with Cu: ZIF-8/KZ3TTz heterojunction. Attributed to the formation of a staggered type II heterostructure between KZ3TTz and Cu: ZIF-8 semiconductors, the Cu: ZIF-8/KZ3TTz heterojunction exhibited stable and significant cathode PEC response. Impressively, selective MIP film was grown on the surface of Cu: ZIF-8/KZ3TTz/GCE by electro-polymerization of 2,2-Dimethyl-5-(3-thienyl)-1,3-dioxane-4,6-dione (DTDD) in the presence of E. coli. After removing E. coli, more electrons were transferred to the electrolyte solution through the imprinting cavity on the MIP film, which was eliminated by O in the electrolyte, causing further enhancement of the cathode PEC response. On the contrary, when the imprinted cavity was filled with E. coli, the cathodic PEC response gradually decreased due to steric hindrance effect. The sensor showed excellent linearity in the range of 10 to 10 CFU/mL with a detection limit of 4.09 CFU/mL (S/N = 3). This strategy offered a novel approach for pathogenic bacteria detection in food safety and environmental monitoring.
在此，通过将聚噻吩薄膜与 Cu:ZIF-8/KZ3TTz 异质结结合，设计了一种信号稳定的分子印迹光电化学（MIP-PEC）传感平台，用于灵敏检测大肠杆菌。由于 KZ3TTz 和 Cu:ZIF-8 半导体之间形成了交错型 II 型异质结，Cu:ZIF-8/KZ3TTz 异质结表现出稳定且显著的阴极 PEC 响应。令人印象深刻的是，在存在大肠杆菌的情况下，通过在 Cu:ZIF-8/KZ3TTz/GCE 表面上进行 2,2-二甲基-5-(3-噻吩基)-1,3-二氧戊环-4,6-二酮（DTDD）的电聚合，在 Cu:ZIF-8/KZ3TTz 表面上生长出选择性的 MIP 薄膜。在去除大肠杆菌后，更多的电子通过 MIP 薄膜上的印迹腔转移到电解质溶液中，这些电子被电解质中的 O 消除，从而进一步增强了阴极 PEC 响应。相反，当印迹腔被大肠杆菌填充时，由于空间位阻效应，阴极 PEC 响应逐渐降低。该传感器在 10 到 10 CFU/mL 的范围内表现出优异的线性，检测限为 4.09 CFU/mL（S/N = 3）。该策略为食品安全和环境监测中致病菌的检测提供了一种新方法。
