Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan 430062, China; Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Biosens Bioelectron. 2019 Feb 15;127:57-63. doi: 10.1016/j.bios.2018.11.040. Epub 2018 Dec 6.
A rapid and ultrasensitive molecularly imprinted photoelectrochemical (MIP-PEC) sensing platform based on ITO electrode modified with GO-CdS heterojunction was prepared for ultrasensitive measure of fumonisin B (FB). CdS quantum dots (QDs) were combined with a suitable amount of graphene oxide (GO) to form a heterojunction to enhance signal response with accurately calculating energy levels (VB/CB or HOMO/LUMO). The MIP-PEC sensor was successful fabricated after MIP was immobilized on the electrode with the basis of these results. In the phosphate buffer solution (PBS), it was clearly observed that the non-elution MIP-PEC sensor had almost no photocurrent response, which was due to the slower electron transfer speed. When the MIP-PEC sensor is eluted in ethanol, its photocurrent response was significantly restored, that was because the fact that the template molecules were washed away, and electron donors entered the holes and accelerated the electron transfer. Its photocurrent response was reduced because of holes blocked when the MIP-PEC sensor was hatched in the template molecules culture fluid. This phenomenon fully showed that the MIP-PEC sensor can specifically detect the target. Thus, The work has a linear range from 0.01 to 1000 ng mL with a detection limit of 4.7 pg mL for FB. Furthermore, the fabricated MIP-PEC sensor will confirm the actual application.
基于 ITO 电极修饰的 GO-CdS 异质结的快速灵敏分子印迹光电流（MIP-PEC）传感平台用于灵敏测定伏马菌素 B（FB）。将适量的石墨烯氧化物（GO）与 CdS 量子点（QDs）结合形成异质结，以增强信号响应并准确计算能级（VB/CB 或 HOMO/LUMO）。在此基础上，将 MIP 固定在电极上，成功制备了 MIP-PEC 传感器。在磷酸盐缓冲溶液（PBS）中，未洗脱的 MIP-PEC 传感器几乎没有光电流响应，这是因为电子转移速度较慢。当 MIP-PEC 传感器在乙醇中洗脱时，其光电流响应显著恢复，这是因为模板分子被洗脱，电子供体进入空穴并加速电子转移。当 MIP-PEC 传感器在模板分子培养液中孵化时，由于空穴被阻塞，其光电流响应降低。这一现象充分表明 MIP-PEC 传感器可以特异性地检测目标。因此，该工作的线性范围为 0.01 至 1000ngmL，检测限为 4.7pgmL 的 FB。此外，所制备的 MIP-PEC 传感器将用于实际应用的确认。
