Engineering Technology Research Center of Henan Province for MEMS Manufacturing and Applications, School of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, People's Republic of China.
Industrial Technology Research Institute, Zhengzhou University, Zhengzhou 450001, People's Republic of China.
Biofabrication. 2024 Aug 12;16(4). doi: 10.1088/1758-5090/ad6794.
In contrast to traditional two-dimensional cell-culture conditions, three-dimensional (3D) cell-culture models closely mimic complexconditions. However, constructing 3D cell culture models still faces challenges. In this paper, by using micro/nano fabrication method, including lithography, deposition, etching, and lift-off, we designed magnetic nanostructures resembling a crown of thorns. This magnetic crown of thorns (MCT) nanostructure enables the isolation of cells that have endocytosed magnetic particles. To assess the utility of this nanostructure, we used high-flux acquisition of Jurkat cells, an acute-leukemia cell line exhibiting the native phenotype, as an example. The novel structure enabled Jurkat cells to form spheroids within just 30 min by leveraging mild magnetic forces to bring together endocytosed magnetic particles. The size, volume, and arrangement of these spheroids were precisely regulated by the dimensions of the MCT nanostructure and the array configuration. The resulting magnetic cell clusters were uniform in size and reached saturation after 1400 s. Notably, these cell clusters could be easily separated from the MCT nanostructure through enzymatic digestion while maintaining their integrity. These clusters displayed a strong proliferation rate and survival capabilities, lasting for an impressive 96 h. Compared with existing 3D cell-culture models, the approach presented in this study offers the advantage of rapid formation of uniform spheroids that can mimicmicroenvironments. These findings underscore the high potential of the MCT in cell-culture models and magnetic tissue enginerring.
与传统的二维细胞培养条件相比，三维（3D）细胞培养模型更能模拟复杂的条件。然而，构建 3D 细胞培养模型仍然面临挑战。在本文中，我们通过使用微/纳米制造方法，包括光刻、沉积、刻蚀和剥离，设计了类似于荆棘冠的磁性纳米结构。这种磁性荆棘冠（MCT）纳米结构能够分离内吞磁性颗粒的细胞。为了评估这种纳米结构的实用性，我们以高通量获取 Jurkat 细胞（一种表现出天然表型的急性白血病细胞系）为例。通过利用温和的磁场力将内吞的磁性颗粒聚集在一起，这种新结构使 Jurkat 细胞在短短 30 分钟内形成球体。这些球体的大小、体积和排列方式可以通过 MCT 纳米结构的尺寸和阵列配置精确调节。得到的磁性细胞簇大小均匀，在 1400 秒后达到饱和。值得注意的是，这些细胞簇可以通过酶消化很容易地从 MCT 纳米结构中分离出来，同时保持其完整性。这些细胞簇表现出很强的增殖率和生存能力，持续了令人印象深刻的 96 小时。与现有的 3D 细胞培养模型相比，本研究中提出的方法具有快速形成可模拟微环境的均匀球体的优势。这些发现突显了 MCT 在细胞培养模型和磁性组织工程中的巨大潜力。
