使用培养的哺乳动物细胞进行衰老测定的简要指南。

Brief guide to senescence assays using cultured mammalian cells.

机构信息

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, South Korea.

School of Biological Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea.

出版信息

Mol Cells. 2024 Sep;47(9):100102. doi: 10.1016/j.mocell.2024.100102. Epub 2024 Jul 23.

DOI:10.1016/j.mocell.2024.100102

PMID:39053732

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11374973/

Abstract

Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.

摘要

细胞衰老是与生物体衰老和许多慢性疾病相关的重要生物学过程。在这里，我们提供了一份关于哺乳动物衰老测定的简要指南，包括细胞周期停滞的测量、细胞形态变化、衰老相关β-半乳糖苷酶（SA-β-gal）染色以及衰老相关分泌表型（SASP）的表达。这项工作对于具有细胞衰老测定最低专业知识的生物学家将是有用的。