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E. coli recA protein possesses a strand separating activity on short duplex DNAs.

作者信息

Bianchi M, Riboli B, Magni G

出版信息

EMBO J. 1985 Nov;4(11):3025-30. doi: 10.1002/j.1460-2075.1985.tb04039.x.

DOI:10.1002/j.1460-2075.1985.tb04039.x
PMID:3905387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554614/
Abstract

RecA protein was found to catalyze the dissociation of the strands of a DNA substrate consisting of a 20-nucleotide primer annealed to circular single-stranded M13mp DNA. The strand separation reaction requires ATP hydrolysis and the presence of single-stranded DNA flanking the duplex DNA region to be unwound. RecA-catalyzed strand separation is effective only for very short duplexes, not exceeding 30 bp, and is not stimulated by single-stranded DNA-binding protein. These results are consistent with the ability of recA protein to disrupt regions of secondary structure in single-stranded DNA and to incorporate large non-homologies into heteroduplex DNA.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/4f6aaccc533b/emboj00276-0293-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/7499119cc87f/emboj00276-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/3cb269f4b627/emboj00276-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/549cd8fb4477/emboj00276-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/d4398ed7f5f5/emboj00276-0293-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/4f6aaccc533b/emboj00276-0293-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/7499119cc87f/emboj00276-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/3cb269f4b627/emboj00276-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/549cd8fb4477/emboj00276-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/d4398ed7f5f5/emboj00276-0293-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc55/554614/4f6aaccc533b/emboj00276-0293-c.jpg

相似文献

1
E. coli recA protein possesses a strand separating activity on short duplex DNAs.
EMBO J. 1985 Nov;4(11):3025-30. doi: 10.1002/j.1460-2075.1985.tb04039.x.
2
recA protein binding to the heteroduplex product of DNA strand exchange.RecA蛋白与DNA链交换的异源双链产物结合。
J Biol Chem. 1987 Jan 25;262(3):1337-43.
3
3' homologous free ends are required for stable joint molecule formation by the RecA and single-stranded binding proteins of Escherichia coli.3'同源自由末端是大肠杆菌的RecA和单链结合蛋白形成稳定连接分子所必需的。
Proc Natl Acad Sci U S A. 1987 Feb;84(3):690-4. doi: 10.1073/pnas.84.3.690.
4
Stable binding of recA protein to duplex DNA. Unraveling a paradox.
J Biol Chem. 1987 Jan 25;262(3):1326-36.
5
RecA-mediated strand exchange reactions between duplex DNA molecules containing damaged bases, deletions, and insertions.RecA介导的含损伤碱基、缺失和插入的双链DNA分子之间的链交换反应。
J Biol Chem. 1988 May 25;263(15):7431-6.
6
Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA.在同源重组中突触前细丝形成后通过荧光共振能量转移检测到的三个机制步骤。从RecA释放寡核苷酸异源双链体产物需要ATP水解。
Biochemistry. 1998 Aug 18;37(33):11692-706. doi: 10.1021/bi980646s.
7
Stable DNA heteroduplex formation catalyzed by the Escherichia coli RecA protein in the absence of ATP hydrolysis.在无ATP水解的情况下，由大肠杆菌RecA蛋白催化的稳定DNA异源双链体形成。
Proc Natl Acad Sci U S A. 1990 Jan;87(1):21-5. doi: 10.1073/pnas.87.1.21.
8
Radioprobing of a RecA-three-stranded DNA complex with iodine 125: evidence for recognition of homology in the major groove of the target duplex.用碘125对RecA三链DNA复合物进行放射性探测：靶双链体大沟中同源性识别的证据。
J Mol Biol. 2000 Jun 9;299(3):629-40. doi: 10.1006/jmbi.2000.3770.
9
Evidence for a four-strand exchange catalyzed by the RecA protein.RecA蛋白催化四链交换的证据。
Biochemistry. 2000 Dec 12;39(49):15272-81. doi: 10.1021/bi001704o.
10
Formation of covalently closed heteroduplex DNA by the combined action of gyrase and RecA protein.通过促旋酶和RecA蛋白的联合作用形成共价闭合异源双链DNA。
EMBO J. 1984 Sep;3(9):2159-64. doi: 10.1002/j.1460-2075.1984.tb02106.x.

引用本文的文献

1
Helicase-like functions in phosphate loop containing beta-alpha polypeptides.含磷酸环的β-α多肽中的解旋酶样功能。
Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). doi: 10.1073/pnas.2016131118.
2
RecA filament sliding on DNA facilitates homology search.RecA细丝在DNA上滑动有助于同源性搜索。
Elife. 2012 Dec 13;1:e00067. doi: 10.7554/eLife.00067.
3
DNA stretching by bacterial initiators promotes replication origin opening.细菌引发酶拉伸 DNA 促进复制起始点打开。

本文引用的文献

1
Homologous pairing in genetic recombination: recA protein makes joint molecules of gapped circular DNA and closed circular DNA.遗传重组中的同源配对：RecA蛋白使缺口环状DNA和闭环状DNA形成联合分子。
Cell. 1980 May;20(1):223-35. doi: 10.1016/0092-8674(80)90250-0.
2
recA protein promotes homologous-pairing and strand-exchange reactions between duplex DNA molecules.RecA蛋白促进双链DNA分子之间的同源配对和链交换反应。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2100-4. doi: 10.1073/pnas.78.4.2100.
3
By searching processively RecA protein pairs DNA molecules that share a limited stretch of homology.
Nature. 2011 Oct 2;478(7368):209-13. doi: 10.1038/nature10455.
4
Crystal structure of reverse gyrase: insights into the positive supercoiling of DNA.反向回旋酶的晶体结构：对DNA正超螺旋的见解。
EMBO J. 2002 Feb 1;21(3):418-26. doi: 10.1093/emboj/21.3.418.
5
A novel property of the RecA nucleoprotein filament: activation of double- stranded DNA for strand exchange in trans.RecA核蛋白丝的一种新特性：双链DNA的反式链交换激活。
Genes Dev. 1999 Aug 1;13(15):2005-16. doi: 10.1101/gad.13.15.2005.
6
Structure and function of RecA-DNA complexes.RecA-脱氧核糖核酸复合物的结构与功能
Experientia. 1994 Mar 15;50(3):192-203. doi: 10.1007/BF01924002.
7
Homologous recombination intermediates between two duplex DNA catalysed by human cell extracts.人细胞提取物催化的两条双链DNA之间的同源重组中间体。
Nucleic Acids Res. 1987 Jul 24;15(14):5643-55. doi: 10.1093/nar/15.14.5643.
8
Perturbed chromosomal replication in recA mutants of Escherichia coli.大肠杆菌recA突变体中染色体复制紊乱。
J Bacteriol. 1988 Jun;170(6):2549-54. doi: 10.1128/jb.170.6.2549-2554.1988.
9
Properties of the duplex DNA-dependent ATPase activity of Escherichia coli RecA protein and its role in branch migration.大肠杆菌RecA蛋白双链DNA依赖性ATP酶活性的特性及其在分支迁移中的作用。
Proc Natl Acad Sci U S A. 1987 May;84(10):3127-31. doi: 10.1073/pnas.84.10.3127.
10
DNA helicase activity of SV40 large tumor antigen.SV40大肿瘤抗原的DNA解旋酶活性
EMBO J. 1986 Aug;5(8):1939-44. doi: 10.1002/j.1460-2075.1986.tb04447.x.
通过逐步搜索，RecA蛋白会配对具有有限同源片段的DNA分子。
Cell. 1983 Sep;34(2):647-54. doi: 10.1016/0092-8674(83)90397-5.
4
Unwinding associated with synapsis of DNA molecules by recA protein.RecA蛋白介导的DNA分子联会相关的解旋过程。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1256-60. doi: 10.1073/pnas.80.5.1256.
5
Homologous pairing in genetic recombination. Purification and characterization of Escherichia coli recA protein.遗传重组中的同源配对。大肠杆菌recA蛋白的纯化与特性分析。
J Biol Chem. 1981 Jul 25;256(14):7557-64.
6
Homologous pairing of single-stranded circular DNAs catalyzed by recA protein.由recA蛋白催化的单链环状DNA的同源配对。
Nucleic Acids Res. 1984 Aug 10;12(15):6127-39. doi: 10.1093/nar/12.15.6127.
7
Characterization of the Escherichia coli SSB-113 mutant single-stranded DNA-binding protein. Cloning of the gene, DNA and protein sequence analysis, high pressure liquid chromatography peptide mapping, and DNA-binding studies.
J Biol Chem. 1984 Jan 25;259(2):805-14.
8
Mechanism of the concerted action of recA protein and helix-destabilizing proteins in homologous recombination.RecA蛋白与解旋稳定蛋白在同源重组中的协同作用机制。
Proc Natl Acad Sci U S A. 1984 May;81(9):2757-61. doi: 10.1073/pnas.81.9.2757.
9
Synapsis promoted by Ustilago rec1 protein.黑粉菌rec1蛋白促进联会。
Cell. 1984 Mar;36(3):593-8. doi: 10.1016/0092-8674(84)90338-6.
10
Insertions, deletions and mismatches in heteroduplex DNA made by recA protein.RecA蛋白形成的异源双链DNA中的插入、缺失和错配。
Cell. 1983 Dec;35(2 Pt 1):511-20. doi: 10.1016/0092-8674(83)90185-x.