RecA蛋白促进双链DNA分子之间的同源配对和链交换反应。
recA protein promotes homologous-pairing and strand-exchange reactions between duplex DNA molecules.
作者信息
West S C, Cassuto E, Howard-Flanders P
出版信息
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2100-4. doi: 10.1073/pnas.78.4.2100.
Abstract
Purified recA protein, product of the recA+ gene, promotes homologous pairing between intact covalent circular duplex DNA and circular single-stranded DNA carrying a short hybridized fragment [West, S. C., Cassuto, E. & Howard-Flanders, P. (1981) Nature (London) 290, 29-33.]. In this paper we investigate the interaction of duplex fragments with circular single-stranded DNA carrying the hybridized fragment and find that recA protein promotes an efficient strand-exchange reaction between interacting DNA molecules. The exchange is dependent upon linear duplex DNA fragments that are homologous to, but extend beyond, the short fragment present on the hybridized DNA substrate. The reactions require stoichiometric amounts of recA protein and the presence of ATP.
摘要
recA+基因的产物——纯化的RecA蛋白，可促进完整的共价闭环双链DNA与携带短杂交片段的环状单链DNA之间的同源配对[韦斯特，S.C.，卡苏托，E. & 霍华德-弗兰德斯，P.（1981年）《自然》（伦敦）290, 29 - 33页]。在本文中，我们研究了双链片段与携带杂交片段的环状单链DNA之间的相互作用，发现RecA蛋白可促进相互作用的DNA分子之间高效的链交换反应。这种交换依赖于与杂交DNA底物上存在的短片段同源但又超出其范围的线性双链DNA片段。这些反应需要化学计量的RecA蛋白和ATP的存在。
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Proc Natl Acad Sci U S A. 1981 Apr;78(4):2100-4. doi: 10.1073/pnas.78.4.2100.
2
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Initiation of genetic recombination: homologous pairing between duplex DNA molecules promoted by recA protein.遗传重组的起始：由recA蛋白促进的双链DNA分子之间的同源配对。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3962-6. doi: 10.1073/pnas.77.7.3962.
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Recognition of duplex DNA containing single-stranded regions by recA protein.RecA蛋白对含有单链区域的双链DNA的识别。
Proc Natl Acad Sci U S A. 1980 May;77(5):2569-73. doi: 10.1073/pnas.77.5.2569.
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E. coli recA protein-directed cleavage of phage lambda repressor requires polynucleotide.大肠杆菌RecA蛋白介导的噬菌体λ阻遏物切割需要多核苷酸。
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