Department for Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Oral Microbiology and Biomaterials Laboratory, Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
Dent Mater. 2024 Oct;40(10):1515-1523. doi: 10.1016/j.dental.2024.07.004. Epub 2024 Jul 25.
To investigate the cytotoxicity on human dental pulp cells (HDPCs) and Streptococcus mutans (S.mutans) biofilm formation on universal resin composite cements (UCs).
Three UCs (RelyX Universal, 3 M Oral Care - RXU; Panavia SA Cement Universal, Kuraray Noritake - PSAU; SoloCem, Coltene - SCM) and one 'gold-standard' multi-step cement (Panavia V5, Kuraray Noritake - PV5) were used following two polymerization protocols (light-cured - LC; self-cured - SC). Cytotoxicity (MTT) tests were performed after 1, 3 and 7 days of direct contact. Carboxy-2',7'-dichlorodihydrofluorescein diacetate was used to detect the release of reactive oxygen species (ROS), and interleukin 6 (IL-6) expression was analyzed by IL-6 proquantum high sensitivity immunoassay. S. mutans biofilms were grown on UCs samples in a bioreactor for 24 h, then adherent viable biomass was assessed using MTT assay. For microbiological procedures, half of UCs samples underwent accelerated aging. Data were statistically analyzed (α = 0.05).
The highest cytotoxicity was observed for PSAU SC, RXU SC, and PV5 SC at day 1, then for SC RXU after 3 days, and SC PSAU, LC PV5 and SCM after 1-week (p < 0.05). There was no increase in IL-6 expression after 1 day, while it increased depending on the group at 3 and 7 days. The highest ROS expression after 12 h was recorded for PSAU SC, PV5 SC and PV5 LC. Biofilm formation was as follows: RXU > > PSAU = PV5 > SCM, while light-curing systematically decreased biofilm formation (≈-33 %). Aging leveled out differences between UCs and between polymerization protocols.
The choice of cement brand, rather than category, and polymerization protocol influence cell viability and microbiological behavior. Light-curing is beneficial for reducing the harmful pulpal effect that UCs may possess.
研究通用树脂水门汀（UCs）对人牙髓细胞（HDPCs）的细胞毒性和变形链球菌（S.mutans）生物膜形成的影响。
采用三种通用树脂水门汀（RelyX Universal，3M 口腔护理 - RXU；Panavia SA 通用水泥，Kuraray Noritake - PSAU；SoloCem，Coltene - SCM）和一种“金标准”多步水泥（Panavia V5，Kuraray Noritake - PV5），按两种聚合方案（光固化 - LC；自固化 - SC）进行测试。直接接触 1、3 和 7 天后进行细胞毒性（MTT）试验。用羧基-2'，7'-二氯二氢荧光素二乙酸酯检测活性氧（ROS）的释放，用 IL-6 proquantum 高灵敏度免疫分析检测白细胞介素 6（IL-6）的表达。在生物反应器中培养 UCs 样本上的 S. mutans 生物膜 24 小时，然后用 MTT 测定法评估附着的活生物质。对于微生物程序，一半的 UCs 样本进行了加速老化。数据进行了统计学分析（α=0.05）。
在第 1 天，PSAU SC、RXU SC 和 PV5 SC 的细胞毒性最高，然后在第 3 天 SC RXU 的细胞毒性最高，第 1 周后 SC PSAU、LC PV5 和 SCM 的细胞毒性最高（p<0.05）。在第 1 天，IL-6 表达没有增加，而在第 3 和第 7 天，表达则取决于组。在 12 小时时，PSAU SC、PV5 SC 和 PV5 LC 记录到最高的 ROS 表达。生物膜形成情况如下：RXU>PSAU=PV5>SCM，而光固化则系统地降低了生物膜的形成（≈-33%）。老化使 UCs 之间以及聚合方案之间的差异趋于一致。
水泥品牌的选择而不是类别以及聚合方案会影响细胞活力和微生物行为。光固化有利于减少 UCs 可能对牙髓产生的有害影响。
