Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
Vet Anaesth Analg. 2024 Sep-Oct;51(5):558-565. doi: 10.1016/j.vaa.2024.06.012. Epub 2024 Jun 29.
To describe an ultrasound-guided approach to the sciatic nerve and the distribution of nerve staining using two injectate volumes of dye in barred owls.
Descriptive, randomized, assessor-blinded, cadaveric study.
Twelve barred owl cadavers.
The sciatic nerve was visualized with a 13-6 MHz linear ultrasound probe placed on the medial aspect of the proximal femur in barred owl cadavers. A needle was inserted at the proximal caudal thigh, and cranially advanced in-plane until the tip was immediately adjacent and ventral to the sciatic nerve. Eleven owls were injected bilaterally with a 1:1 solution of 1% methylene blue and 0.5% ropivacaine, with high-volume (HV; 0.4 mL kg) and low-volume (LV; 0.2 mL kg) treatments. Nerve staining ≥ 1 cm circumferentially was determined by dissection 15 minutes post-injection. The coelom was then opened to inspect for aberrant dye spread. In one barred owl cadaver, a 1:1 solution of 1% methylene blue and 74% ioversol contrast was injected, and computed tomography was performed immediately before and 15 minutes after injection for evaluation of injectate spread.
The HV treatment (0.4 mL kg) resulted in successful nerve staining in 100% of injections, whereas the LV treatment (0.2 mL kg) resulted in successful nerve staining in 91% of injections. There was no leakage of injectate into undesired locations (e.g. coelomic cavity) in any cadaver.
In barred owls, the sciatic nerve can be visualized with ultrasound and injecting a ropivacaine/dye solution under ultrasound guidance successfully stained the sciatic nerve in the majority of cases. This technique holds potential for providing analgesia distal to the stifle joint; however, further investigations are necessary to evaluate its practical application in a clinical setting.
描述一种在条纹猫头鹰中使用两种染料注射量对坐骨神经和神经染色分布进行超声引导的方法。
描述性、随机、评估者盲法、尸体研究。
12 只条纹猫头鹰尸体。
在条纹猫头鹰尸体的股骨近端内侧放置 13-6MHz 线性超声探头，可观察到坐骨神经。将一根针插入大腿近尾端，在平面内向头侧推进，直到针尖紧邻并位于坐骨神经腹侧。11 只猫头鹰双侧注射 1%亚甲蓝和 0.5%罗哌卡因的 1:1 溶液，高容量（HV；0.4mL/kg）和低容量（LV；0.2mL/kg）处理。注射后 15 分钟通过解剖确定神经染色≥1cm 周长。然后打开体腔检查异常染料扩散情况。在一只条纹猫头鹰尸体中，注射了 1%亚甲蓝和 74%碘海醇的 1:1 溶液，注射前后立即进行 CT 检查，以评估注射剂的扩散情况。
HV 处理（0.4mL/kg）的 100%注射成功染色坐骨神经，而 LV 处理（0.2mL/kg）的 91%注射成功染色坐骨神经。在任何尸体中，都没有注射剂漏入不需要的位置（例如体腔）。
在条纹猫头鹰中，超声可观察到坐骨神经，在超声引导下注射罗哌卡因/染料溶液可成功染色大多数情况下的坐骨神经。该技术有望为膝关节以下提供镇痛作用；然而，需要进一步的研究来评估其在临床环境中的实际应用。
