Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China.
College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.
J Agric Food Chem. 2024 Aug 7;72(31):17306-17316. doi: 10.1021/acs.jafc.4c04094. Epub 2024 Jul 25.
Overexpression of carboxyl/cholinesterase (CCE) genes has been reported to be associated with many cases of pesticide resistance in arthropods. However, it has been rarely documented that genes participate in spirodiclofen resistance in . In previous research, we found that spirodiclofen resistance is related to increased P450 and CCE enzyme activities in . In this study, we identified two genes, and , which were significantly upregulated in spirodiclofen-resistant strain and after exposure to spirodiclofen. RNA interference of and increased the spirodiclofen susceptibility in . In vitro metabolism indicated that CCE3 and CCE5 could interact with spirodiclofen, but metabolites were detected only in the CCE3 treatment. Our results indicated that CCE3 participates in spirodiclofen resistance through direct metabolism, and CCE5 may be involved in the spirodiclofen resistance by passive binding and sequestration, which provides new insights into spirodiclofen resistance in .
已报道过羧酸酯酶/胆碱酯酶（Carboxyl/cholinesterase，CCE）基因的过表达与昆虫中的许多杀虫剂抗性有关。然而，关于基因参与螺螨酯抗性的报道却很少。在之前的研究中，我们发现螺螨酯抗性与斜纹夜蛾中 P450 和 CCE 酶活性的增加有关。在本研究中，我们鉴定了两个基因 和 ，它们在螺螨酯抗性品系中以及暴露于螺螨酯后显著上调。 和 的 RNA 干扰增加了 对螺螨酯的敏感性。体外代谢表明 CCE3 和 CCE5 可以与螺螨酯相互作用，但仅在 CCE3 处理中检测到代谢物。我们的结果表明，CCE3 通过直接代谢参与螺螨酯抗性，而 CCE5 可能通过被动结合和隔离参与螺螨酯抗性，这为斜纹夜蛾中螺螨酯抗性提供了新的见解。
