Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, National Citrus Engineering Research Center, Chongqing 400712, China.
Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, National Citrus Engineering Research Center, Chongqing 400712, China; Chongqing Institute for Food and Drug Control, Key Laboratory of Condiment Supervision Technology for State Market Regulation, Chongqing 401121, PR China.
Pestic Biochem Physiol. 2024 Jun;202:105952. doi: 10.1016/j.pestbp.2024.105952. Epub 2024 May 11.
The citrus red mite, Panonychus citri, is one of the most notorious and devastating citrus pests around the world that has developed resistance to multiple chemical acaricides. In previous research, we found that spirodiclofen-resistant is related to overexpression of P450, CCE, and ABC transporter genes in P. citri. However, the regulatory mechanisms of these detoxification genes are still elusive. This study identified all hormone receptor 96 genes of P. citri. 8 PcHR96 genes contained highly conserved domains. The expression profiles showed that PcHR96h was significantly upregulated in spirodiclofen resistant strain and after exposure to spirodiclofen. RNA interference of PcHR96h decreased expression of detoxification genes and increased spirodiclofen susceptibility in P. citri. Furthermore, molecular docking, heterologous expression, and drug affinity responsive target stability demonstrated that PcHR96h can interact with spirodiclofen in vitro. Our research results indicate that PcHR96h plays an important role in regulating spirodiclofen susceptibility and provides theoretical support for the resistance management of P. citri.
柑橘全爪螨，又称柑橘红蜘蛛，是世界上最臭名昭著、极具破坏力的柑橘害虫之一，它已经对多种化学杀螨剂产生了抗性。在之前的研究中，我们发现，螺螨酯抗性与柑橘全爪螨中 P450、CCE 和 ABC 转运蛋白基因的过度表达有关。然而，这些解毒基因的调控机制仍不清楚。本研究鉴定了柑橘全爪螨的所有激素受体 96 基因。8 个 PcHR96 基因包含高度保守的结构域。表达谱显示，螺螨酯抗性品系和暴露于螺螨酯后 PcHR96h 的表达显著上调。PcHR96h 的 RNA 干扰降低了柑橘全爪螨解毒基因的表达，并增加了对螺螨酯的敏感性。此外，分子对接、异源表达和药物亲和反应靶标稳定性实验表明，PcHR96h 可以在体外与螺螨酯相互作用。我们的研究结果表明，PcHR96h 在调节螺螨酯敏感性方面起着重要作用，为柑橘全爪螨的抗性管理提供了理论支持。
