Auzan C, Devaux C, Houot A M, Laboulandine I, Corvol P, Menard J, Chrambach A
Mol Cell Endocrinol. 1985 Sep;42(2):175-83. doi: 10.1016/0303-7207(85)90105-4.
Enzymatically inactive human renin from chorionic cells in culture is significantly distinct in polyacrylamide gel electrophoresis (pH 8.17, 0 degree C) from active human kidney renin. The inactive renin is larger and more basic than the active renin; their molecular weights derived from gel electrophoretic retardation coefficients relate as 47.5/35.3 kDa, their valences (net protons/molecule) as 2.14/1.85. In gel electrofocusing conducted in a mixture of simple buffers, both inactive and active renins exhibit 2 components at the steady-state. The molecular size and basicity of inactive renin are consistent with the hypothesis that it may be a precursor (prorenin), although the possibility that it is an inhibitor complex cannot be ruled out.
培养的绒毛膜细胞中无酶活性的人肾素在聚丙烯酰胺凝胶电泳（pH 8.17，0℃）中与活性人肾素明显不同。无活性肾素比活性肾素更大且碱性更强；从凝胶电泳延迟系数得出的它们的分子量之比为47.5/35.3 kDa，它们的化合价（每分子净质子数）之比为2.14/1.85。在简单缓冲液混合物中进行的凝胶等电聚焦中，无活性和活性肾素在稳态时均显示出2种成分。无活性肾素的分子大小和碱性与它可能是前体（肾素原）的假设一致，尽管不能排除它是抑制剂复合物的可能性。
