Kohnert K D, Wilke B, Schmidt S, Schäfer H, Reiher K, Hahn von Dorsche H
Mol Cell Endocrinol. 1985 Nov;43(1):95-103. doi: 10.1016/0303-7207(85)90046-2.
Sand rats were fed either a vegetable (vegetable group) or a standard pellet diet. After 14-16 weeks, the normoglycemic subgroup selected (pellet group) from the animals that had been maintained on the standard diet showed a modest increase in body weight. Plasma immunoreactive insulin levels were not significantly increased, but glucose-stimulated insulin release was elevated from islets isolated from sand rats of the pellet group. Insulin biosynthesis was estimated in vitro by measuring [3H]leucine incorporation into (pro)insulin at 1.5 or 15 mmol/l glucose. The rate of (pro)insulin biosynthesis was elevated only at 15 mmol/l glucose in islets from those normoglycemic sand rats fed the pellet diet when compared with islets from the vegetable group. Specific insulin-degrading activity, as determined by measuring degradation of 125I-labeled insulin, was also increased for islets from the pellet group. The metabolic state of these sand rats is thus associated with normoglycemia in vivo, and increased stimulated rates of insulin biosynthesis and degradation in pancreatic islets in vitro.
将沙鼠分为两组，分别喂食蔬菜（蔬菜组）或标准颗粒饲料。14 - 16周后，从食用标准饮食的动物中挑选出的血糖正常亚组（颗粒饲料组）体重有适度增加。血浆免疫反应性胰岛素水平没有显著升高，但从颗粒饲料组沙鼠分离的胰岛中，葡萄糖刺激的胰岛素释放增加。通过测量[3H]亮氨酸在1.5或15 mmol/l葡萄糖浓度下掺入（前）胰岛素的量，在体外估计胰岛素生物合成。与蔬菜组的胰岛相比，仅在喂食颗粒饲料的血糖正常沙鼠的胰岛中，当葡萄糖浓度为15 mmol/l时，（前）胰岛素生物合成速率升高。通过测量125I标记胰岛素的降解来确定的特异性胰岛素降解活性，在颗粒饲料组的胰岛中也增加。因此，这些沙鼠的代谢状态与体内血糖正常相关，并且在体外胰腺胰岛中胰岛素生物合成和降解的刺激速率增加。
