负责葡萄糖阻遏的酵母己糖激酶PII基因（HXK2）的一级结构。

The primary structure of the yeast hexokinase PII gene (HXK2) which is responsible for glucose repression.

作者信息

Fröhlich K U, Entian K D, Mecke D

出版信息

Gene. 1985;36(1-2):105-11. doi: 10.1016/0378-1119(85)90074-5.

Abstract

The nucleotide sequence of the Saccharomyces cerevisiae gene encoding the glycolytic isoenzyme hexokinase PII (HXK2), which is responsible for triggering glucose repression, has been determined. The reading frame was identified by comparison with the N-terminal undecameric amino acid (aa) sequence, determined previously [Schmidt and Colowick, Arch. Biochem. Biophys. 158 (1973) 458-470]. The codon sequence was not random, with 82.1% of the aa specified by only 25 codons. The structural gene sequence corresponded to 1455 bp, coding for 485 aa residues, corresponding to the Mr of 53 800 for the HXK2 monomer. Five initiation regions spanning 162 bp and three termination sites spanning 29 bp were detected. Sequences with similarities to a 5'-TATAAA-3' sequence were located 24-39 bp upstream of each initiation region. The most pronounced initiation region corresponded to the 5'-TATAAA-3' sequence at position -152. Two of the minor initiation sites were inside the coding sequence in front of two ATG codons.

摘要

已确定酿酒酵母中编码糖酵解同工酶己糖激酶PII（HXK2）的基因的核苷酸序列，该酶负责引发葡萄糖阻遏。通过与先前确定的N端十一聚体氨基酸（aa）序列进行比较，确定了阅读框[施密特和科洛维克，《生物化学与生物物理学报》158（1973）458 - 470]。密码子序列并非随机，82.1%的氨基酸仅由25个密码子指定。结构基因序列对应1455 bp，编码485个氨基酸残基，对应HXK2单体的分子量为53800。检测到五个跨度为162 bp的起始区域和三个跨度为29 bp的终止位点。与5'-TATAAA-3'序列相似的序列位于每个起始区域上游24 - 39 bp处。最明显的起始区域对应于-152位的5'-TATAAA-3'序列。两个次要起始位点位于两个ATG密码子之前的编码序列内。