Reha-Krantz L J
Gene. 1985;38(1-3):275-6. doi: 10.1016/0378-1119(85)90229-x.
The Escherichia coli JM105 strain was constructed as a sup0 strain to facilitate the cloning of selected recombinants (Yanisch-Perron et al., 1985). In our work with bacteriophage T4, we observed that several T4 am mutants could grow on JM105. To characterize the suppressor activity of JM105, we tested the growth of several T4 am mutants on a variety of E. coli suppressor-containing strains.
大肠杆菌JM105菌株构建为sup0菌株,以促进所选重组体的克隆(Yanisch-Perron等人,1985年)。在我们对噬菌体T4的研究中,我们观察到几个T4 am突变体能够在JM105上生长。为了表征JM105的抑制活性,我们测试了几个T4 am突变体在多种含大肠杆菌抑制子的菌株上的生长情况。