Edelmann P, Martin R, Gallant J
Mol Gen Genet. 1987 May;207(2-3):517-8. doi: 10.1007/BF00331625.
Nonsense suppression by supE44 has been examined in a collection of 14 T4 gene 22 and gene 23 UAG mutants, for which the precise gene location is known. In concordance with previous studies, UAG followed by a pyrimidine was inefficiently suppressed. However, among positions with similar 3' nucleotides, there was considerable variation in suppression efficiency. The competition between supE44 and Release Factor 1 (RF 1) was also investigated following the introduction of a multicopy RF 1 plasmid. An inverse relationship between the efficiency of suppression and RF 1 competition was observed.
已在一组14个T4基因22和基因23的UAG突变体中检测了supE44介导的无义抑制作用，这些突变体的精确基因位置是已知的。与先前的研究一致，UAG后接嘧啶时抑制效率较低。然而，在具有相似3'核苷酸的位置之间，抑制效率存在相当大的差异。在引入多拷贝RF 1质粒后，还研究了supE44与释放因子1（RF 1）之间的竞争。观察到抑制效率与RF 1竞争之间呈负相关。
