De Vos R, De Wolf-Peeters C, van den Oord J J, Desmet V
Hepatology. 1985 Nov-Dec;5(6):1071-5. doi: 10.1002/hep.1840050602.
Major histocompatibility complex products Class I (HLA Class I) antigens are not expressed on the surface of normal human hepatocytes but become so in pathological conditions. The purpose of this study was to specify the ultrastructural topography of HLA Class I antigens expression. Nine human liver specimens, known from light microscopic investigation to display membranous positivity for HLA Class I antigens, were processed for immunoelectronmicroscopy using monoclonal anti-HLA Class I in an indirect immunoperoxidase procedure. HLA Class I antigens were detected on the basolateral membrane of hepatocytes and bile duct cells; some cisternae of the endoplasmic reticulum were also positive. The membranes of normal bile canaliculi of hepatocytes and the apical border of bile duct cells were negative. In one case of presumably drug-induced cholestasis, abnormal cholestatic canaliculi displayed HLA Class I antigens. These results indicate that HLA Class I antigens are synthesized by the hepatocytes and bile duct cells and incorporated into the plasma membrane; the basolateral expression follows the pattern as in other polarized cells. The expression in cholestatic canaliculi suggests a disturbed polarity of the hepatocyte.
主要组织相容性复合体I类产物（HLA I类）抗原在正常人类肝细胞表面不表达，但在病理状态下会表达。本研究的目的是明确HLA I类抗原表达的超微结构定位。对9例经光镜检查已知显示HLA I类抗原膜阳性的人类肝脏标本，采用间接免疫过氧化物酶法，用单克隆抗HLA I类抗体进行免疫电镜处理。在肝细胞和胆管细胞的基底外侧膜上检测到HLA I类抗原；内质网的一些池也呈阳性。肝细胞正常胆小管的膜和胆管细胞的顶端边界呈阴性。在1例可能由药物引起的胆汁淤积病例中，异常的胆汁淤积性胆小管显示有HLA I类抗原。这些结果表明，HLA I类抗原由肝细胞和胆管细胞合成并整合到质膜中；基底外侧的表达遵循与其他极化细胞相同的模式。胆汁淤积性胆小管中的表达提示肝细胞极性紊乱。
