Barbatis C, Woods J, Morton J A, Fleming K A, McMichael A, McGee J O
Gut. 1981 Dec;22(12):985-91. doi: 10.1136/gut.22.12.985.
The distribution of HLA class I antigens was studied in 42 liver biopsies and eight necropsies by an immunoperoxidase technique employing a monoclonal antibody which reacts with the heavy chains of class I (A, B, C) HLA antigens. In normal liver HLA class I antigens could not be detected on hepatocyte cell membranes or cytoplasm; these antigens were present on the cell membrane of bile duct epithelium, on sinusoidal lining cells, fibroblasts, and blood vessel endothelium. However, in all patients with acute alcoholic hepatitis, most cases of primary biliary cirrhosis and some cases of chronic active hepatitis HLA class I antigens were detectable focally or diffusely on the cell membrane of hepatocytes; in two cases of acute viral hepatitis (non-A, non-B) HLA class I antigens were present in granular form in the cytoplasm of all hepatocytes. These findings may be relevant to the prolonged survival of liver allografts in man and other species and in the pathogenesis of some liver diseases.
采用一种与I类（A、B、C）HLA抗原重链发生反应的单克隆抗体，通过免疫过氧化物酶技术，对42例肝活检组织和8例尸检组织中的HLA I类抗原分布进行了研究。在正常肝脏中，未在肝细胞细胞膜或细胞质上检测到HLA I类抗原；这些抗原存在于胆管上皮细胞膜、窦状隙衬里细胞、成纤维细胞和血管内皮上。然而，在所有急性酒精性肝炎患者、大多数原发性胆汁性肝硬化病例以及一些慢性活动性肝炎病例中，可在肝细胞细胞膜上局灶性或弥漫性检测到HLA I类抗原；在2例急性病毒性肝炎（非甲非乙型）中，所有肝细胞的细胞质中均以颗粒形式存在HLA I类抗原。这些发现可能与人类和其他物种肝移植的长期存活以及某些肝脏疾病的发病机制有关。
