Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Master Program of Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2024 Jun;14(6):1467-1475. doi: 10.5455/OVJ.2024.v14.i6.15. Epub 2024 Jun 30.
The Trichuris eggs are collected from naturally infected sheep. Natural antihelmintics such as herbal medicines are needed as an alternative, such as natural compounds from endemic plants.
This present study aims to evaluate the ovicidal activity and cytotoxicity effects of ethanolic extract of (EECL) and (EECS) as a biological anthelmintic against the egg of .
The eggs are collected from naturally infected sheep. CMC-Na solution 1% was used as a control. The treatments were 0.12% EECL; 0.24% EECL; 0.15% EECS; 0.30% EECS; a combination of 0.12% EECL and 0.30% EECS; a combination of 0.24% EECL; and 0.15% EECS. Ovicidal activity testing by microscopic examination of eggs treated using different concentrations of EECL extract, EECS, and a combination of them. They were exposed for various times (7, 14, 21, and 28 days) and incubated at room temperature.
The study shows that a combination of extract and tea extract exhibits good ovicidal anthelmintic activity against . in sheep. Cytotoxicity examination using the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) test. Based on MTT data processed using regression analysis, the obtained LC from the administration of EECL, EECS, and a combination of both in a ratio of 1:1, 2:2, 1:2, and 2:1. The combination of EECL extract and EECS with the highest concentration produced cell viability of 28.46%, 17.25%, 56.01%, and 46.47%, respectively.
It can be concluded that the most cytotoxic ingredient is found in the combination of EECL and EECS (2:2) at 17.25% and the safest is in the ratio (1:2) at 56.01%.
从自然感染的绵羊中收集旋毛虫卵。需要天然驱虫药作为替代品，例如来自地方特有植物的天然化合物。
本研究旨在评估旋毛虫卵的杀卵活性和细胞毒性作用，并评估从地方性植物中提取的天然化合物（EECL 和 EECS）作为一种生物驱虫药对的卵的影响。
从自然感染的绵羊中收集卵。使用 CMC-Na 溶液 1%作为对照。处理方法为 0.12%EECL；0.24%EECL；0.15%EECS；0.30%EECS；0.12%EECL 和 0.30%EECS 的组合；0.24%EECL 和 0.15%EECS 的组合。通过显微镜检查用不同浓度的 EECL 提取物、EECS 及其组合处理的卵，进行杀卵活性测试。它们暴露在不同的时间（7、14、21 和 28 天）下，并在室温下孵育。
研究表明，旋毛虫提取物和茶提取物的组合对绵羊的具有良好的杀卵驱虫活性。使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物（MTT）试验进行细胞毒性检查。基于使用回归分析处理的 MTT 数据，从 EECL、EECS 以及它们 1:1、2:2、1:2 和 2:1 的组合的给药中获得 LC。EECL 提取物和 EECS 的组合以最高浓度产生的细胞活力分别为 28.46%、17.25%、56.01%和 46.47%。
可以得出结论，最具细胞毒性的成分存在于 EECL 和 EECS（2:2）的组合中，为 17.25%，最安全的是（1:2）的比例，为 56.01%。
