Yilmaz Filiz, Aydemi R Selma, Yilmaz Bayram, Ilgen Orkun, Kurt Sefa, Baykara Başak
Hitit University, Training and Research Hospital, IVF Center, Corum, Turkey.
Dokuz Eylul University, Faculty of Medicine, Department of Histology and Embryology, Izmir, Turkey.
Iran J Basic Med Sci. 2024;27(9):1180-1186. doi: 10.22038/IJBMS.2024.76513.16557.
Using histopathological and immunohistochemical methods, we aimed to examine the dose-dependent effects of chronic caffeine consumption on the recovery of burn wounds in an rat model.
Forty-five rats were randomly assigned to a high-dose group (20 mg/kg per day for eight weeks; n=15), a low-dose group (10 mg/kg per day for eight weeks; n=15), or a control group (n=15). The burn model was created in rats. The groups were separated into three subgroups (n=5) based on the day after injury (7, 14, or 21 day). The wound area, wound closure percentage, and histopathological and immunohistochemical reactivity were evaluated.
Successful wound healing was noted in rats treated with low doses of caffeine, similar to the control group. Pathology revealed low re-epithelization, low inflammation, and high granulation in the high-dose group. In addition, there was a significant difference between the control and high-dose groups regarding the immunohistochemical reactivity of αVβ3 integrin, vascular endothelial growth factor (VEGF), and matrix metalloproteinase 9 (MMP-9) (0.05).
We demonstrated that chronic caffeine consumption in rats adversely affects the recovery process of wounds in a dose-dependent manner. This effect may occur through delayed wound healing via the molecules MMP-9, αVβ3 integrin, and VEGF. Treatment that modulates these molecules can lead to enhanced and quicker recovery of damaged skin in coffee lovers.
运用组织病理学和免疫组织化学方法，我们旨在研究大鼠模型中慢性咖啡因摄入对烧伤创面愈合的剂量依赖性影响。
45只大鼠被随机分为高剂量组（每天20毫克/千克，持续8周；n = 15）、低剂量组（每天10毫克/千克，持续8周；n = 15）或对照组（n = 15）。在大鼠身上建立烧伤模型。根据伤后天数（7、14或21天）将各组再分为三个亚组（n = 5）。评估创面面积、创面闭合百分比以及组织病理学和免疫组织化学反应性。
低剂量咖啡因治疗的大鼠创面愈合良好，与对照组相似。病理学检查显示高剂量组再上皮化程度低、炎症反应低且肉芽组织增生高。此外，对照组与高剂量组在αVβ3整合素、血管内皮生长因子（VEGF）和基质金属蛋白酶9（MMP - 9）的免疫组织化学反应性方面存在显著差异（P < 0.05）。
我们证明大鼠慢性咖啡因摄入以剂量依赖性方式对创面愈合过程产生不利影响。这种影响可能通过MMP - 9、αVβ3整合素和VEGF等分子导致创面愈合延迟。调节这些分子的治疗可使咖啡爱好者受损皮肤的愈合增强且加快。
