Song Hang, Chen Ruoyu, Ren Liyuan, Zeng Yinuo, Sun Junfeng, Tong Shanbao
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
iScience. 2024 Jun 17;27(7):110269. doi: 10.1016/j.isci.2024.110269. eCollection 2024 Jul 19.
Collective studies have demonstrated that transcranial ultrasound stimulation (TUS) can elicit activation in hemodynamics, implying its potential in treating cerebral or peripheral vessel-related malfunction. The theory for hemodynamic response to TUS is neurovascular coupling (NVC) following the ultrasound-induced cellular (de)polarization. However, it was not conclusive due to the co-existence of the pathway of direct ultrasound-vessel interactions. This study thus aims to investigate and provide direct evidence for NVC pathway in a rodent model of TUS by inhibiting neural activity with sodium valproate (VPA), a GABAergic agent. Twenty Sprague-Dawley rats were randomly assigned to VPA and Saline groups. Microelectrode and optical imaging were utilized to record local field potential and relative cerebral blood flow (rCBF) during baseline, before, and after TUS periods. We found the attenuated neural activity was associated with reduced rCBF responses. These results provided direct evidence for the presence of NVC pathway in hemodynamic modulation by TUS.
多项研究表明，经颅超声刺激（TUS）可引起血流动力学激活，这意味着其在治疗脑或外周血管相关功能障碍方面具有潜力。TUS引起血流动力学反应的理论是超声诱导细胞（去）极化后的神经血管耦合（NVC）。然而，由于直接超声-血管相互作用途径的共存，这一点并不确凿。因此，本研究旨在通过用γ-氨基丁酸能药物丙戊酸钠（VPA）抑制神经活动，在TUS啮齿动物模型中研究并为NVC途径提供直接证据。将20只Sprague-Dawley大鼠随机分为VPA组和生理盐水组。在基线期、TUS期间之前和之后，利用微电极和光学成像记录局部场电位和相对脑血流量（rCBF）。我们发现神经活动减弱与rCBF反应降低有关。这些结果为TUS在血流动力学调节中存在NVC途径提供了直接证据。
