Gao Tian, Wang Qiong, Sun Huarui, Liu Yang, Li Jitao, He Yuying
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Biology (Basel). 2024 Jun 30;13(7):488. doi: 10.3390/biology13070488.
The rapid development of the mariculture industry has been hindered by limited coastal aquaculture space. To utilize the abundant inland saline-alkaline water, we studied the physiological effects of high carbonate alkalinity stress and high pH stress on . The study employed quantitative proteomics by tandem mass tag (TMT) and non-targeted metabolomics analysis using a liquid chromatograph mass spectrometer (LC-MS) to understand the physiological and biochemical adaptive mechanisms of the hepatopancreas of in response to saline-alkaline stress at the molecular level. We designed two stress groups as follows: a high carbonate alkalinity (CA) group and a combined high carbonate alkalinity and high pH (CP) group. The study found that the protein and metabolic profiles of the two stress groups were changed, and the CP group, which was exposed to dual stresses, incurred more severe damage to the hepatopancreas compared to that of the CA group. After exposure to CA and CP, the hepatopancreas of showed significant alterations in 455 proteins and 50 metabolites, and 1988 proteins and 272 metabolites, respectively. In addition, upregulated the level of energy metabolism in the hepatopancreas to defend against osmotic imbalance caused by CA or CP stress, which was demonstrated by the significant upregulation of important proteins and metabolites in glycolysis, pyruvate metabolism, TCA cycle, and fatty acid oxidation. Additionally, pattern recognition receptors, the phenol oxidase system, and various immune-related metabolic enzymes and metabolites were also affected. The immune homeostasis of was affected by the alteration of the antioxidant system following exposure to CA or CP. These findings provide valuable information for saline-alkaline water cultivation practices.
沿海水产养殖空间有限阻碍了海水养殖业的快速发展。为了利用丰富的内陆盐碱水，我们研究了高碳酸盐碱度胁迫和高pH胁迫对[具体对象未提及]的生理影响。该研究采用串联质谱标签（TMT）定量蛋白质组学和液相色谱质谱联用仪（LC-MS）进行非靶向代谢组学分析，以从分子水平了解[具体对象未提及]肝胰腺应对盐碱胁迫的生理生化适应机制。我们设计了两个胁迫组，如下：高碳酸盐碱度（CA）组和高碳酸盐碱度与高pH联合（CP）组。研究发现，两个胁迫组的蛋白质和代谢谱均发生了变化，与CA组相比，遭受双重胁迫的CP组对肝胰腺造成的损伤更严重。暴露于CA和CP后，[具体对象未提及]的肝胰腺分别有455种蛋白质和50种代谢物、1988种蛋白质和272种代谢物出现显著变化。此外，[具体对象未提及]上调了肝胰腺中的能量代谢水平，以抵御由CA或CP胁迫引起的渗透失衡，这通过糖酵解、丙酮酸代谢、三羧酸循环和脂肪酸氧化中重要蛋白质和代谢物的显著上调得到证明。此外，模式识别受体、酚氧化酶系统以及各种免疫相关代谢酶和代谢物也受到影响。暴露于CA或CP后，抗氧化系统的改变影响了[具体对象未提及]的免疫稳态。这些发现为[具体对象未提及]的盐碱水养殖实践提供了有价值的信息。
