Kondratenko Albina A, Tovpeko Dmitry V, Volov Daniil A, Kalyuzhnaya Lidia I, Chernov Vladimir E, Glushakov Ruslan I, Sirotkina Maria Y, Zemlyanoy Dmitry A, Bildyug Natalya B, Chebotarev Sergey V, Alexander-Sinclair Elga I, Nashchekin Alexey V, Belova Aleksandra D, Grigoriev Alexey M, Kirsanova Ludmila A, Basok Yulia B, Sevastianov Victor I
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia.
Department of Histology and Embryology, St. Petersburg State Pediatric Medical University, 194100 St. Petersburg, Russia.
Biomimetics (Basel). 2024 Jul 3;9(7):405. doi: 10.3390/biomimetics9070405.
The umbilical cord is a material that enhances regeneration and is devoid of age-related changes in the extracellular matrix (ECM). The aim of this work was to develop a biodegradable scaffold from a decellularized human umbilical cord (UC-scaffold) to heal full-thickness wounds. Decellularization was performed with 0.05% sodium dodecyl sulfate solution. The UC-scaffold was studied using morphological analysis methods. The composition of the UC-scaffold was studied using immunoblotting and Fourier transform infrared spectroscopy. The adhesion and proliferation of mesenchymal stromal cells were investigated using the LIVE/DEAD assay. The local reaction was determined by subcutaneous implantation in mice ( = 60). A model of a full-thickness skin wound in mice ( = 64) was used to assess the biological activity of the UC-scaffold. The proposed decellularization method showed its effectiveness in the umbilical cord, as it removed cells and retained a porous structure, type I and type IV collagen, TGF-β3, VEGF, and fibronectin in the ECM. The biodegradation of the UC-scaffold in the presence of collagenase, its stability during incubation in hyaluronidase solution, and its ability to swell by 1617 ± 120% were demonstrated. Subcutaneous scaffold implantation in mice showed gradual resorption of the product in vivo without the formation of a dense connective tissue capsule. Epithelialization of the wound occurred completely in contrast to the controls. All of these data suggest a potential for the use of the UC-scaffold.
脐带是一种可促进再生的材料，其细胞外基质（ECM）不存在与年龄相关的变化。本研究的目的是从脱细胞人脐带（UC支架）开发一种可生物降解的支架，用于愈合全层伤口。使用0.05%十二烷基硫酸钠溶液进行脱细胞处理。采用形态学分析方法对UC支架进行研究。利用免疫印迹和傅里叶变换红外光谱研究UC支架的组成。使用活/死细胞检测法研究间充质基质细胞的黏附和增殖。通过在小鼠皮下植入（n = 60）来确定局部反应。使用小鼠全层皮肤伤口模型（n = 64）评估UC支架的生物活性。所提出的脱细胞方法在脐带中显示出有效性，因为它去除了细胞，并在ECM中保留了多孔结构、I型和IV型胶原蛋白、转化生长因子-β3（TGF-β3）、血管内皮生长因子（VEGF）和纤连蛋白。证明了UC支架在胶原酶存在下的生物降解、在透明质酸酶溶液中孵育期间的稳定性以及其膨胀1617±120%的能力。在小鼠皮下植入支架显示该产品在体内逐渐吸收，未形成致密结缔组织囊。与对照组相比，伤口完全发生了上皮化。所有这些数据表明UC支架具有应用潜力。
