Ribeiro Ricardo Faria, Mata Victor Barboza da, Tomaselli Lucas de Oliveira, Simionato Anselmo Agostinho, Santos Emerson de Souza, Faria Adriana Cláudia Lapria, Rodrigues Renata Cristina Silveira, do Nascimento Cássio
Department of Dental Materials and Prostheses, School of Dentistry of Ribeirao Preto, University of São Paulo, Ribeirão Preto 14040-904, Brazil.
Department of Clinical Analysis, Toxicology, and Food Science, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirão Preto 14040-903, Brazil.
Dent J (Basel). 2024 Jul 19;12(7):226. doi: 10.3390/dj12070226.
The objective of this study was to evaluate microbial leakage by means of genome counts, through the implant-abutment interface in dental implants with different Morse taper abutments. Fifty-six samples were prepared and divided in four groups: CMC TB (14 Cylindrical Implants-14 TiBase Abutments), CMX TB (14 Conical Implants-14 TiBase Abutments), CMX PU (14 Conical Implants-14 Universal Abutment) and CMX U (14 Tapered Implants-14 UCLA Abutments). Assemblies had their interface submerged in saliva as the contaminant. Samples were subjected either to thermomechanical cycling (2 × 10 mechanical cycles with frequency of 5 Hz and load of 120 N simultaneously with thermal cycles of 5-55 °C) or thermal cycling (5-55 °C). After cycling, the contents from the inner parts of assemblies were collected and analyzed using the Checkerboard DNA-DNA hybridization technique. Significant differences in the total genome counts were found after both thermomechanical or thermal cycling: CMX U > CMX PU > CMX TB > CMC TB. There were also significant differences in individual bacterial counts in each of the groups ( < 0.05). Irrespective of mechanical cycling, the type of abutment seems to influence not only the total microbial leakage through the interface, but also seems to significantly reflect differences considering individual target species.
本研究的目的是通过基因组计数，评估不同莫氏锥度基台的牙种植体中种植体-基台界面的微生物渗漏情况。制备了56个样本，并将其分为四组：CMC TB（14个圆柱形种植体-14个钛基台）、CMX TB（14个锥形种植体-14个钛基台）、CMX PU（14个锥形种植体-14个通用基台）和CMX U（14个锥形种植体-14个UCLA基台）。将组件的界面浸没在作为污染物的唾液中。样本分别进行热机械循环（2×10个机械循环，频率为5 Hz，负载为120 N，同时进行5-55°C的热循环）或热循环（5-55°C）。循环后，收集组件内部的内容物，并使用棋盘式DNA-DNA杂交技术进行分析。在热机械循环或热循环后，总基因组计数均发现显著差异：CMX U>CMX PU>CMX TB>CMC TB。各组的单个细菌计数也存在显著差异（<0.05）。无论是否进行机械循环，基台类型似乎不仅会影响通过界面的总微生物渗漏，而且似乎在考虑单个目标物种时也能显著反映差异。
