Hsieh Hsi-Lung, Yu Ming-Chin, Chang Yu-Chia, Wu Yi-Hsuan, Huang Kuo-Hsiung, Tsai Ming-Ming
Graduate Institute of Health Industry Technology, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan.
Department of Neurology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan.
Curr Issues Mol Biol. 2024 Jul 9;46(7):7303-7323. doi: 10.3390/cimb46070433.
Gastric inflammation-related disorders are commonly observed digestive system illnesses characterized by the activation of proinflammatory cytokines, particularly tumor necrosis factor-α (TNF-α). This results in the induction of cyclooxygenase-2 (COX-2)/prostaglandin E (PEG) and matrix metallopeptidase-9 (MMP-9). These factors contribute to the pathogenesis of gastric inflammation disorders. We examined the preventive effects of Thunb. ethanol extract (Lj-EtOH) on gastric inflammation induced by TNF-α in normal human gastric mucosa epithelial cells (GES-1). The GES-1 cell line was used to establish a model that simulated the overexpression of COX-2/PGE and MMP-9 proteins induced by TNF-α to examine the anti-inflammatory properties of Lj extracts. The results indicated that Lj-EtOH exhibits significant inhibitory effects on COX-2/PEG and MMP-9 activity, attenuates cell migration, and provides protection against TNF-α-induced gastric inflammation. The protective effects of Lj-EtOH are associated with the modulation of COX-2/PEG and MMP-9 through the activation of TNFR-ERK 1/2 signaling pathways as well as the involvement of c-Fos and nuclear factor kappa B (NF-κB) signaling pathways. Based on our findings, Lj-EtOH exhibits a preventive effect on human gastric epithelial cells. Consequently, it may represent a novel treatment for the management of gastric inflammation.
胃炎症相关疾病是常见的消化系统疾病，其特征在于促炎细胞因子的激活，特别是肿瘤坏死因子-α（TNF-α）。这导致环氧化酶-2（COX-2）/前列腺素E（PEG）和基质金属蛋白酶-9（MMP-9）的诱导。这些因素促成胃炎症疾病的发病机制。我们研究了地锦草乙醇提取物（Lj-EtOH）对正常人胃黏膜上皮细胞（GES-1）中TNF-α诱导的胃炎症的预防作用。使用GES-1细胞系建立一个模拟由TNF-α诱导的COX-2/PGE和MMP-9蛋白过表达的模型，以研究Lj提取物的抗炎特性。结果表明，Lj-EtOH对COX-2/PEG和MMP-9活性具有显著的抑制作用，减弱细胞迁移，并提供针对TNF-α诱导的胃炎症的保护作用。Lj-EtOH的保护作用与通过激活TNFR-ERK 1/2信号通路以及c-Fos和核因子κB（NF-κB）信号通路对COX-2/PEG和MMP-9的调节有关。基于我们的研究结果，Lj-EtOH对人胃上皮细胞具有预防作用。因此，它可能代表一种治疗胃炎症的新方法。
