Sousa Gabriela C, Carvalho Marcos G, Fonseca-Alves Carlos E, Souza Fabiana F
Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, Unesp, Botucatu 18618-687, São Paulo, Brazil.
Department of Small Animal Clinical Sciences, Virginia Maryland College of Veterinary Medicine, Blacksburg, VA 24061, USA.
Curr Issues Mol Biol. 2024 Jul 22;46(7):7745-7768. doi: 10.3390/cimb46070459.
This study investigated serum extracellular vesicles (EVs) in bitches with mammary neoplasms, in order to understand their size, shape, and concentration, as well as their association with tumor malignancy. Thirty bitches were categorized into control ( = 10), mammary tumor grades I and II (GI, = 13), and grade III (GII, = 7). Serum was separated from blood collected during mastectomy, and EVs were isolated using size exclusion chromatography. The analysis revealed no significant differences in EV concentrations among groups, with similar concentrations for control, GI, and GII. Ninety-one proteins were identified in EV-enriched samples, with six showing varied abundance across groups. Notably, keratin 18 was highly abundant in GI, while sushi domain-containing protein, EvC ciliary subunit 2, and the joining chain of multimeric IgM and IgA were increased in GII. Additionally, protocadherin 17 and albumin were upregulated in both GI and GII. ROC curves identified potential biomarkers for differentiating tumor grades. Enrichment pathway analysis revealed AFP gene upregulation in the GI. Mass spectrometry proteomics data were deposited in Mendeley Data. The study provides valuable insights into serum EV characterization in bitches, suggesting keratin 18 and protocadherin 17 as potential biomarkers for canine mammary neoplasia, with implications for future diagnostic and therapeutic strategies.
本研究调查了患有乳腺肿瘤的母犬血清中的细胞外囊泡（EVs），以了解其大小、形状和浓度，以及它们与肿瘤恶性程度的关联。30只母犬被分为对照组（n = 10）、乳腺肿瘤I级和II级组（GI，n = 13）以及III级组（GII，n = 7）。在乳房切除术期间采集血液并分离血清，使用尺寸排阻色谱法分离EVs。分析显示各组之间的EV浓度无显著差异，对照组、GI组和GII组的浓度相似。在富含EV的样本中鉴定出91种蛋白质，其中6种在各组中的丰度有所不同。值得注意的是，角蛋白18在GI组中高度丰富，而含寿司结构域蛋白、EvC纤毛亚基2以及多聚体IgM和IgA的连接链在GII组中增加。此外，原钙黏蛋白17和白蛋白在GI组和GII组中均上调。ROC曲线确定了区分肿瘤分级的潜在生物标志物。富集通路分析显示GI组中AFP基因上调。质谱蛋白质组学数据已存入Mendeley Data。该研究为母犬血清EV的特征提供了有价值的见解，表明角蛋白18和原钙黏蛋白17作为犬乳腺肿瘤的潜在生物标志物，对未来的诊断和治疗策略具有重要意义。
