Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
UCD School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Dublin, Ireland.
PLoS One. 2022 Sep 20;17(9):e0274261. doi: 10.1371/journal.pone.0274261. eCollection 2022.
Diffuse large B-cell lymphomas (DLBCLs) are the most common lymphoproliferative diseases in dogs. DLBCL diagnosis to date has relied on histopathological analysis; however liquid biopsies have gained attention in recent years as a source of diagnostic and prognostic information. Liquid biopsies can be a source of circulating DNA, miRNA, circulating tumour cells or extracellular vesicles (EVs). In this study EVs were isolated from the plasma of healthy dogs, and dogs with lymphoma, and adenocarcinoma by iodixanol density gradient centrifugation. These EVs were positive for the EV markers CD63 and TSG101 and the pan-B cell markers CD79a, CD21, CD45, CD20. NTA analysis revealed that the DLBCL and adenocarcinoma dogs had elevated plasma EVs relative to the healthy dogs. Furthermore, the modal size of lymphoma EVs had decreased relative to healthy dogs while adenocarcinoma EVs were unchanged. This study demonstrates that the plasma EV population is altered in canine lymphoma patients in a manner similar to previous studies on human lymphomas. The similar changes to the EV population in dogs, together with the similar pathological features and treatment protocols in canine and human non-Hodgkin lymphomas would make dogs a good comparative model for studying the role of EVs in DLBCL development and progression.
弥漫性大 B 细胞淋巴瘤（DLBCL）是犬类最常见的淋巴组织增生性疾病。迄今为止，DLBCL 的诊断依赖于组织病理学分析；然而，近年来液体活检作为一种诊断和预后信息的来源受到了关注。液体活检可以作为循环 DNA、miRNA、循环肿瘤细胞或细胞外囊泡（EVs）的来源。在这项研究中，通过碘克沙醇密度梯度离心法从健康犬、淋巴瘤犬和腺癌犬的血浆中分离 EVs。这些 EVs 对 EV 标志物 CD63 和 TSG101 以及泛 B 细胞标志物 CD79a、CD21、CD45、CD20 呈阳性。NTA 分析显示，与健康犬相比，DLBCL 和腺癌犬的血浆 EV 水平升高。此外，淋巴瘤 EV 的模态大小相对于健康犬减小，而腺癌 EV 则保持不变。本研究表明，犬类淋巴瘤患者的血浆 EV 群体发生了改变，与之前对人类淋巴瘤的研究相似。犬类 EV 群体的相似变化，加上犬类和人类非霍奇金淋巴瘤在病理特征和治疗方案上的相似性，使犬类成为研究 EV 在 DLBCL 发生和发展中的作用的良好比较模型。
