Guan Liwen, Wang Xianzhong, Wan Sijing, Wang Yuanyuan, Zhang Xinyu, Wang Shigui, Li Can, Tang Bin
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China.
Insects. 2024 Jul 5;15(7):502. doi: 10.3390/insects15070502.
Cytochrome P450 monooxygenases (CYP), crucial detoxification enzymes in insects, are involved in the metabolism of endogenous substances as well as the activation and degradation of exogenous compounds. In this study, was utilized to investigate the roles of and genes influencing in the trehalose metabolism pathway under high-CO stress. By predicting the functional sequences of and genes and analyzing their spatiotemporal expression patterns, it was discovered that both genes belong to the CYP3 group and exhibit high expression levels during the larval stage, decreasing during the pupal stage, while showing high expression in the fatty body, intestine, and malpighian tubules. Furthermore, following the knockdown of and genes in combination with treating larvae with 75% CO, it was observed that larval mortality increased, and glycogen content significantly decreased, while trehalose content increased significantly. Additionally, membrane-bound trehalase enzyme activity declined, TPS gene expression was significantly upregulated, GS gene expression was significantly downregulated, and ATP content showed a marked decrease. In conclusion, CYP genes are critical responsive genes of to high CO levels, potentially impacting the insect's resistance to carbon dioxide through their involvement in the synthesis or breakdown of the carbohydrate metabolism pathway. These findings could serve as a theoretical basis for the utilization of novel pesticides in low-oxygen grain storage techniques and offer new insights for environmentally friendly pest control strategies in grain storage.
细胞色素P450单加氧酶（CYP）是昆虫体内关键的解毒酶，参与内源性物质的代谢以及外源化合物的活化和降解。在本研究中，利用[具体内容缺失]来研究[具体基因缺失]基因在高CO胁迫下对海藻糖代谢途径的影响。通过预测[具体基因缺失]基因的功能序列并分析其时空表达模式，发现这两个基因都属于CYP3组，在幼虫阶段表达水平较高，在蛹期下降，而在脂肪体、肠道和马氏管中表达较高。此外，在[具体基因缺失]基因敲低并结合用75% CO处理幼虫后，观察到幼虫死亡率增加，糖原含量显著降低，而海藻糖含量显著增加。此外，膜结合海藻糖酶活性下降，TPS基因表达显著上调，GS基因表达显著下调，ATP含量显著降低。总之，CYP基因是[具体内容缺失]对高CO水平的关键响应基因，可能通过参与碳水化合物代谢途径的合成或分解来影响昆虫对二氧化碳的抗性。这些发现可为低氧粮食储存技术中新型农药的利用提供理论依据，并为粮食储存中的环保害虫防治策略提供新的见解。
