Insulin-Like Regulates Trehalose Metabolism to Tolerate Hypoxia/Hypercapnia in .

RNAi was used to downregulate the expression of insulin-like peptides (), with air-modulation, and high-concentration CO stress, in the larvae of . We assessed the changes in carbohydrate-related content, trehalase activity, and the expression levels of trehalose pathway genes. And pupation, adult emergence, pupation rate, and mortality were assessed. There was a significant change in the expression of in , at a certain concentration of CO. RNAi did not alter the trehalose content significantly, however, the glycogen and glucose content increased significantly. High-concentration CO stress altered the trehalose content and reduced the glycogen and glucose content. The expression levels of and were up-regulated by hypoxia/hypercapnia and combination, with the increase of CO concentration, other trehalase genes begin to respond successively. knockout raised the mortality and reduced the pupation rate and eclosion rate in CO. Understanding the insulin pathway responses to hypoxic stress induced by a high concentration of CO would further elucidate the mechanisms underlying trehalose metabolism in insects.