Cengiz Ziya Ozan, Durmus Ercan, Celik Ilhami, Aktı Ahmet
Oral and Maxillofacial Department, Faculty of Dentistry, Selcuk University, Konya 42250, Turkey.
Department of Histology and Embryology, Faculty of Veterinary medicine, Selcuk University, Konya 42250, Turkey.
J Funct Biomater. 2024 Jul 22;15(7):201. doi: 10.3390/jfb15070201.
The aim of this study was to evaluate the biocompatibility and effectiveness in terms of osseointegration of dental implants composed of novel injectable bone grafts with and without ostrich eggshell particles and membrane protein in rabbit femur.
Sixteen adult male New Zealand rabbits were used in this study. A bone defect was created in each animal's right and left femur, and a dental implant was placed adjacent to the defect. Two graft materials were prepared, one containing the membrane protein and the other not. In two groups, the defects were filled with these materials. In the negative control group (NC, (n:8)), the defects were left empty. A commercial product of biphasic calcium sulfate was used as a positive control material (PC, = 8). The graft groups were defined as the group with the membrane protein (MP+, (n:8)), and without the membrane protein (MP-, n:8). The animals were euthanized at the 12th week after surgery. The samples were investigated using histology, histomorphometry, and micro-computed tomography. Data were statistically analyzed using one-way ANOVA and Tukey's tests ( = 0.05).
Both the PC and MP+ groups had similar newly formed bone areas, and the mean values of these groups were significantly ( < 0.05) higher than those of the MP- and NC groups. The PC group had the highest amount of unresorbed material, while the MP- group had the lowest amount of unresorbed material. The bone-implant contact (BIC) scores of the PC and MP+ groups were significantly higher ( < 0.05) than that of the NC group. The connective tissue area of the PC group was the lowest, which was significantly lower than the other groups ( < 0.05).
The grafts produced are highly biocompatible and also showed osteoproductivity. Their cost-effectiveness and osteoproductive activity require further investigation.
本研究的目的是评估由新型可注射骨移植材料制成的牙科植入物在兔股骨中有无鸵鸟蛋壳颗粒和膜蛋白时的生物相容性以及骨整合效果。
本研究使用了16只成年雄性新西兰兔。在每只动物的左右股骨上制造骨缺损，并在缺损附近植入牙科植入物。制备了两种移植材料，一种含有膜蛋白，另一种不含。在两组中，缺损用这些材料填充。在阴性对照组（NC，n = 8）中，缺损保持为空。一种双相硫酸钙商业产品用作阳性对照材料（PC，n = 8）。移植组定义为含膜蛋白组（MP +，n = 8）和不含膜蛋白组（MP -，n = 8）。动物在手术后第12周安乐死。使用组织学、组织形态计量学和微型计算机断层扫描对样本进行研究。数据使用单因素方差分析和Tukey检验进行统计学分析（α = 0.05）。
PC组和MP +组的新形成骨面积相似，且这些组的平均值显著高于MP -组和NC组（P < 0.05）。PC组的未吸收材料量最高，而MP -组的未吸收材料量最低。PC组和MP +组的骨-植入物接触（BIC）评分显著高于NC组（P < 0.05）。PC组的结缔组织面积最低，显著低于其他组（P < 0.05）。
所制备的移植物具有高度生物相容性，并且还显示出骨生成能力。它们的成本效益和骨生成活性需要进一步研究。
