Švedienė Jurgita, Raudonienė Vita, Mizerienė Goda, Rimšaitė Jolanta, Davenis Sigitas Algis, Ivinskis Povilas
Laboratory of Biodeterioration Research, Nature Research Centre, 08412 Vilnius, Lithuania.
Laboratory of Plant Pathology, Nature Research Centre, 08412 Vilnius, Lithuania.
J Fungi (Basel). 2024 Jun 22;10(7):442. doi: 10.3390/jof10070442.
In this study, yeasts from the gut of larvae were isolated and molecularly identified. It is worth noting that this research provides the first analysis of the gut yeast community in larvae in Lithuania, which is a significant contribution to the field. Two hermit-like L3-praepupa instars were collected from a decaying oak log in Lithuania. The isolation, morphology, biochemistry, and physiology of the yeast isolates were characterized using standards commonly employed in yeast taxonomy studies. The isolates were identified by sequencing the large subunit (26S) rDNA (D1/D2 domain of the LSU). All gut compartments were colonized by the yeast. A total of 45 yeast strains were obtained from the gut of both larvae, with 23 strains originating from Larva 1, 16 strains from Larva 2, and 6 strains from the galleries. According to our identification results of the 45 yeast strains, most of the species were related to , with most of them belonging to the order. Yeasts of the genera , , , , , , , and were present in gut of larvae. Species of the order represented the phylum.
在本研究中，从幼虫肠道中分离出酵母并进行分子鉴定。值得注意的是，本研究首次分析了立陶宛幼虫肠道中的酵母群落，这对该领域具有重要贡献。从立陶宛一根腐烂的橡木原木中采集了两只类似隐士的L3-预蛹龄期幼虫。使用酵母分类学研究中常用的标准对酵母分离株的分离、形态、生物化学和生理学进行了表征。通过对大亚基（26S）rDNA（LSU的D1/D2结构域）进行测序来鉴定分离株。所有肠道区域均被酵母定殖。从两只幼虫的肠道中共获得45株酵母菌株，其中23株来自幼虫1，16株来自幼虫2，6株来自虫道。根据我们对这45株酵母菌株的鉴定结果，大多数物种与……相关，其中大多数属于……目。幼虫肠道中存在……属、……属、……属、……属、……属、……属、……属和……属的酵母。……目的物种代表……门。
