Zhang Jianping, Yang Ke, Tang Wei, Yang Yongjie, Yu Xiaoyue, Lu Yongliang, Yu Liuqing
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Department of Industrial Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
J Fungi (Basel). 2024 Jun 26;10(7):445. doi: 10.3390/jof10070445.
Ophibolin A, a fungal sesterterpene, exerts a pivotal influence in a diverse array of biological processes, encompassing herbicidal, bactericidal, fungicidal, and cytotoxic activities. Sixty genes associated with sesterterpene compound biosynthesis were obtained from via transcriptome sequencing, and those closely linked to ophiobolin A biosynthesis were subsequently filtered. A gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) that catalyzes the first committed step of ophiobolin biosynthesis in the mevalonic acid (MVA) pathway was isolated and characterized using RACE (Rapid Amplification of cDNA Ends) technology from ophiobolin A-producing fungus, . The full-length cDNA of the gene () was 3906 bp and contained a 3474 bp open reading frame (ORF) encoding 1157 amino acids. Sequence analysis revealed that deduced BeHMGR had high homology to the known HMGRs from and . It had a calculated molecular mass of about 124.65 kDa and an isoelectric point (pI) of 6.90. It contained two putative HMG-CoA-binding motifs and two NADP(H)-binding motifs. Induced expression analysis of the gene by methyl jasmonate treatment using quantitative fluorescence PCR showed that it significantly elevated after 3 h of methyl jasmonate treatment, peaked at 6 h, and then gradually decreased. This demonstrates that gene expression is induced by methyl jasmonate.
蛇孢菌素A是一种真菌倍半萜烯，在一系列生物过程中发挥着关键作用，包括除草、杀菌、杀真菌和细胞毒性活性。通过转录组测序从[具体来源未给出]获得了60个与倍半萜烯化合物生物合成相关的基因，随后筛选出了与蛇孢菌素A生物合成密切相关的基因。使用RACE（cDNA末端快速扩增）技术从产蛇孢菌素A的真菌[具体真菌名称未给出]中分离并鉴定了一个编码3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）的基因，该酶催化甲羟戊酸（MVA）途径中蛇孢菌素生物合成的第一步。该基因（BeHMGR）的全长cDNA为3906 bp，包含一个3474 bp的开放阅读框（ORF），编码1157个氨基酸。序列分析表明，推导的BeHMGR与来自[具体物种未给出]和[具体物种未给出]的已知HMGR具有高度同源性。其计算分子量约为124.65 kDa，等电点（pI）为6.90。它包含两个假定的HMG-CoA结合基序和两个NADP（H）结合基序。使用定量荧光PCR通过茉莉酸甲酯处理对BeHMGR基因进行诱导表达分析表明，茉莉酸甲酯处理3小时后其表达显著升高，在6小时达到峰值，然后逐渐下降。这表明BeHMGR基因表达受茉莉酸甲酯诱导。
