Zvonarev Anton, Terentyev Vasily, Zhelifonova Valentina, Antipova Tatiana, Baskunov Boris, Avtukh Aleksander, Abashina Tatiana, Kachalkin Aleksey, Vainshtein Mikhail, Kudryavtseva Anna
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino 142290, Russia.
Institute of Basic Biological Problems, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino 142290, Russia.
J Fungi (Basel). 2024 Jun 29;10(7):463. doi: 10.3390/jof10070463.
Most species are known as endophytes and/or phytopathogens of higher plants and have a worldwide distribution. Recently, information discovered with molecular tools has been also published about the presence of these fungi in the microbiome of truffle fruiting bodies. In the present work, we isolated and identified three strains from truffle fruiting bodies. All isolates were assigned to the same species, , and the strains were deposited in the All-Russian Collection of Microorganisms under accession numbers VKM F-5020, VKM F-5021, and VKM F-5022. To check the possible effects of the isolated strains on the plants, the isolates were used to infect sterile seedlings of Sarepta mustard ( L.). This model infection led to a moderate suppression of the photosynthetic apparatus activity and plant growth. Here, we present characteristics of the isolates: description of the conidial morphology, pigmentation, and composition of the mycelium fatty acids. Overall, this is the first description of the cultures isolated from truffle fruiting bodies. Possible symbiosis of the strains with truffles and their involvement in the cooperative fatty acid production are proposed.
大多数物种是高等植物的内生菌和/或植物病原体，分布于世界各地。最近，利用分子工具发现的有关这些真菌存在于松露子实体微生物群中的信息也已发表。在本研究中，我们从松露子实体中分离并鉴定出三株菌株。所有分离株都被归为同一物种，这些菌株保藏于全俄微生物保藏中心，保藏编号分别为VKM F-5020、VKM F-5021和VKM F-5022。为了检测分离菌株对植物可能产生的影响，将这些分离株用于感染无菌的欧洲油菜（L.）幼苗。这种模型感染导致光合机构活性和植物生长受到适度抑制。在此，我们展示了这些分离株的特征：分生孢子形态、色素沉着以及菌丝体脂肪酸组成的描述。总体而言，这是首次对从松露子实体中分离出的培养物进行描述。我们提出了这些菌株与松露可能存在的共生关系以及它们参与协同脂肪酸生产的情况。
