Joukhadar Radwan, Nižić Nodilo Laura, Lovrić Jasmina, Hafner Anita, Pepić Ivan, Jug Mario
Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10 000 Zagreb, Croatia.
Gels. 2024 Jul 16;10(7):466. doi: 10.3390/gels10070466.
In this study, a functional nanostructured lipid carriers (NLCs)-based hydrogel was developed to repair the damaged epidermal skin barrier. NLCs were prepared via a high-energy approach, using argan oil and beeswax as liquid and solid lipids, respectively, and were loaded with ceramides and cholesterol at a physiologically relevant ratio, acting as structural and functional compounds. Employing a series of surfactants and optimizing the preparation conditions, NLCs of 215.5 ± 0.9 nm in size and a negative zeta potential of -42.7 ± 0.9 were obtained, showing acceptable physical and microbial stability. Solid state characterization by differential scanning calorimetry and X-ray powder diffraction revealed the formation of imperfect crystal NLC-type. The optimized NLC dispersion was loaded into the gel based on sodium hyaluronate and xanthan gum. The gels obtained presented a shear thinning and thixotropic behavior, which is suitable for dermal application. Incorporating NLCs enhanced the rheological, viscoelastic, and textural properties of the gel formed while retaining the suitable spreadability required for comfortable application and patient compliance. The NLC-loaded gel presented a noticeable occlusion effect in vitro. It provided 2.8-fold higher skin hydration levels on the ex vivo porcine ear model than the NLC-free gel, showing a potential to repair the damaged epidermal barrier and nourish the skin actively.
在本研究中，开发了一种基于功能性纳米结构脂质载体（NLCs）的水凝胶，用于修复受损的表皮皮肤屏障。NLCs通过高能方法制备，分别使用摩洛哥坚果油和蜂蜡作为液体和固体脂质，并以生理相关比例负载神经酰胺和胆固醇，作为结构和功能化合物。通过使用一系列表面活性剂并优化制备条件，获得了尺寸为215.5±0.9nm、ζ电位为-42.7±0.9的NLCs，显示出可接受的物理和微生物稳定性。差示扫描量热法和X射线粉末衍射的固态表征揭示了不完全晶体NLC型的形成。将优化后的NLC分散体负载到基于透明质酸钠和黄原胶的凝胶中。所得凝胶呈现剪切变稀和触变性行为，适用于皮肤应用。加入NLCs增强了形成的凝胶的流变学、粘弹性和质地特性，同时保留了舒适应用和患者依从性所需的合适铺展性。负载NLC的凝胶在体外呈现出明显的封闭效果。在离体猪耳模型上，它提供的皮肤水合水平比不含NLC的凝胶高2.8倍，显示出修复受损表皮屏障和积极滋养皮肤的潜力。
