Rehman Saif, Storey Kenneth B
Department of Biology, Carleton Univesrity, Ottawa, ON K1S 5B6, Canada.
Metabolites. 2024 Jul 17;14(7):387. doi: 10.3390/metabo14070387.
The grey tree frog, , survives whole-body freezing for weeks during cold winter months. Survival in a state devoid of available food, water, or oxygen forces a reliance on metabolic rate depression (MRD) and the reprioritization of bodily functions. This study utilizes next-generation sequencing (NGS) and bioinformatic analyses to characterize changes in the microRNAome of . When comparing control to frozen groups, five microRNAs (miRNA) were found to be differentially regulated (miR-143-3p, miR-30e-3p, miR-10a-5p, miR-140-3p, and miR-148a-3p), suggesting that they play key roles in freeze survival. The KEGG and GO analyses of these changes predicted a significant negative enrichment of terms associated with cell proliferation and active metabolism while simultaneously predicting the upregulation of cell signalling terms. These results suggest a fast-acting regulatory role for miRNA in contributing to the reorganization of gene expression and the limitation of energy-expensive processes during MRD in the hind leg skeletal muscle of the frog.
灰树蛙在寒冷的冬季能够全身冻结数周而存活下来。在缺乏可用食物、水或氧气的状态下生存，迫使它依赖代谢率降低（MRD）以及身体功能的重新排序。本研究利用下一代测序（NGS）和生物信息学分析来表征灰树蛙微小RNA组的变化。当将对照组与冷冻组进行比较时，发现有五种微小RNA（miRNA）受到差异调节（miR-143-3p、miR-30e-3p、miR-10a-5p、miR-140-3p和miR-148a-3p），这表明它们在冷冻存活中发挥关键作用。对这些变化的KEGG和GO分析预测，与细胞增殖和活跃代谢相关的术语显著负富集，同时预测细胞信号传导术语上调。这些结果表明，miRNA在青蛙后腿骨骼肌的MRD过程中，对促进基因表达的重组和限制能量消耗大的过程发挥快速调节作用。
